Star Cufflinks Rna Seq

Cufflinks Input. ) • Transcriptome • Whole RNA sequencing • mRNA transcriptome (poly-A selection) • Small RNA analysis (siRNA, snoRNA, lincRNA, etc. Avi Ma'ayan 6,351 views. HISAT is a fast and sensitive spliced alignment program for mapping RNA-seq reads. The methanol-fixation and new processing method was introduced to preserve human peripheral blood mononuclear cells (PBMCs) for single-cell RNA sequencing (scRNA-Seq) analysis on 10× Chromium platform. Next-generation sequencing (NGS) is a high-throughput methodology that enables rapid sequencing of the base pairs in DNA or RNA samples. star Spliced Transcripts Alignment to a Reference (STAR) is a fast RNA-seq read mapper, with support for splice-junction and fusion read detection. VIPER (Visualization Pipeline for RNA-seq analysis) is an analysis workflow that combines some of the most popular tools to take RNA-seq analysis from raw sequencing data, through alignment and quality control, into downstream differential expression and pathway analysis. We demon-strate that it is essential to incorporate biological replicates in the study design. 6 million features Two 25-mer oligonucleotides make up one probe pair of a perfect match (PM) oligo and a. She describes the Cufflinks tool which is a spliced aligner. Both cytoplasmic (5S, 5. See the greatly expanded manual for details. GTF files and the top portion (or all) of a GFF3 file is a reference annotation dataset - describing features on a reference genome/transcriptome. Seq-Star™ rRNA Removal (H/M/R) Kit uses an enzymatic method to remove the highly abundant rRNAs in the total RNA for NGS RNA-seq library preparation. Cufflinks includes a number of tools for analyzing RNA-Seq experiments. Full-length isoform quantification from RNA-Seq is a key goal in transcriptomics analyses and an area of active development. In the following case studies, we outline two pipelines scientists commonly use in the course of RNA-Seq experiments. Here, we used high-throughput RNA sequencing to gain insight into the characterization of the poly-A RNA fraction expressed in pig male gonads. Our data also provide guiding principles for designing RNA-Seq studies of alternative splicing. Note that the knowledge that reverse transcriptase adds terminal Cs as it dissociates from template RNA is important. RAP (RNA-Seq Analysis Pipeline) is a web application implementing a fully automated analysis workflow, designed to integrate in-house developed scripts as well as open source analysis tools into one pipeline (Figure 1). RNA-seq (1) Peter N. The STAR software package performs this task with high levels of accuracy and speed. Article Review: Systematic evaluation of spliced alignment programs for RNA-seq data I was recently asked by a colleague to provide some feedback on a Nature Methods paper by Engström et al. Itis a perfect kit for rapid construction of stranded RNA-seq library. A critical step in RNA-seq data analysis is the alignment of partial transcript reads to a reference genome sequence. Fill the form as follow: Clade: Mammal. Cuffdiff performance assessments. The recent rapid spread of single cell RNA sequencing (scRNA-seq) methods has created a large variety of experimental and computational pipelines for which best practices have not been established, yet. You will need to start R and load the data into R. Transcriptome analysis via RNA-Seq. The pre-compiled STAR executables are located bin/ subdirectory. RNA seq programs included are TopHat, CuffLinks, CuffDiff, CuffMerge, FastQC, and trimming using the FastX toolkit. It is simply a metric, a measurement of RNA transcript fragments as Celsius and Fahrenheit are metrics for measuring temperature. STAR的主程序只有两个:STAR和STARlong。前者用于比对RNA-seq数据,后者是针对于长读长RNA数据。由于同一个程序,又需要做建索引,又需要做序列比对,并且这个程序还支持一系列的输出格式,因此直接用STAR,你会迷失在参数的海洋中。. Sequencing and primary analysis are performed at the Wellcome Trust Sanger Institue. In this week we will cover a lot of the general pipelines people use to analyze specific data types like RNA-seq, GWAS, ChIP-Seq, and DNA Methylation. We will first run Cufflinks on the Tophat condition 1 results. Nat Protoc. , 2011), hydro-tRNAseq (Gogakos et al. Gene expression can be estimated by measuring RNA in the cell. ) • Gene expression profiling for selected target genes • Metagenome • Bulk sequencing of many types of bacteria. org/data-standards/rna-seq/long-rnas/. The Illumina ligation method is a well-know example of this class and is based on sequential ligation of two different adapters. It starts with the RNA-seq experiments that generates reads as we described a couple of lectures ago. Summaries of Results of RNA-Seq Analysis. SCALABLE, REPRODUCIBLE RNA-Seq :7 Seven Bridges Pipelines are ready-to-run bioinformatics workflows. When using paired end RNA-Seq reads, you must take care to supply Cufflinks with the mean and variance on the inner distances between mate pairs. Ours was the first such repository that wasn't limited to human or mouse and included sequencing data from a variety of instruments and library types. Cufflinks then estimates the relative abundances of these transcripts based on how many reads support each one. This is consistent with Cufflinks assembling. Here, we describe a procedure for preparing RNA-Seq libraries from 1 to 4 μg total RNA without poly(A) selection. We then apply and compare the various approaches for differential expression analysis using RNA-Seq. I am trying to use STAR+Cufflinks to do a reference based transcript assembly using unstranded RNA-seq data. DNase I was used to degrade any possible DNA contamination. Lecture 1: Reference genome guided analysis. For small numbers of FastQ files, it can run as a stand-alone interactive program. Cufflinks then estimates the relative abundances of these transcripts based on how many reads support each one. FastQC will give you a series of plots to assess the quality of your Sequecing data. Unlike many other RNA-seq mappers, STAR is not an extension of a short-read DNA mapper, but was developed as a stand-alone C++ code. In contrast to our previous Tang2009 protocol that employed oligo(dT) 24 primers to convert the poly(A)+ mRNAs into cDNAs, SUPeR-seq uses random (AnchorX-T 15 N 6) primers to enable the simultaneous detection of both poly(A)+ and poly(A)- RNA species from a single cell (Fig. b Overlap SNV and Indel calls between STAR and GSNAP alignment algorithm. RSEM is a software package that quantifies gene and isoform abundances from single-end (SE) or paired-end (PE) RNA-Seq data. RSEM (RNA-Seq by Expectation-Maximization) Updates. cummeRbund is an R package. Single-cell RNA sequencing (scRNA-Seq) is an increasingly popular platform to study heterogeneity at the single-cell level. This practical will introduce some popular tools for basic processing of RNA-seq data. The links above point to the R packages directly, and every effort should be made to use the version provided by Bioconductor as it will ensure compatibility with other Bioconductor packages. STARsolo: mapping, demultiplexing and gene quantification for single cell RNA-seq. CummeRbund is an R package that is designed to aid and simplify the task of analyzing Cufflinks RNA-Seq output. You will need to start R and load the data into R. In Cufflinks , an overlap graph is defined over the provided RNA sequence fragments, each represented as a node, in which two fragments are connected by an edge if they are compatible. Robinson Microarrays RNA-seq Alternative splicing mapping cu inks Bipartite A ymetrix Technology The A ymetrix technology uses photolithographic synthesis of oligonucleotides on microarrays. It operates in two stages. (2) A pool of core tissues (e. TopHat: TopHat is a fast splice junction mapper for RNA-Seq reads. Cufflinks assembles transcripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples. In the browser window, select mouse_chr12. The fundamental difficulty stems from the fact that RNA transcripts are long, while RNA-Seq reads are typically short. An in-house RNA-Seq dataset was used and six types of results were generated. VIPER (Visualization Pipeline for RNA-seq analysis) is an analysis workflow that combines some of the most popular tools to take RNA-seq analysis from raw sequencing data, through alignment and quality control, into downstream differential expression and pathway analysis. The materials were prepared for the Next-Generation Sequencing Workshop organized by the Life Science Library Training Courses on 19th Nov 2015 at Academia Sinica. Several new technologies have been introduced for this purpose. RNA-Seq Tutorials • Tutorial 1 - RNA-Seq experiment design and analysis - Instruction on individual software will be provided in other tutorials • Tutorial 2 - Advanced RNA-Seq Analysis topics • Hands-on tutorials - Analyzing human and potato RNA-Seq data using Tophat and Cufflinks in Galaxy. The first four are command line tools on Linux or Mac OS. In this part of the tutorial, you will analyze the results of your RNA -Seq assembly in ArrayStar by using a “quick gene set” and the Gene Table to locate a potential operon structure. It is used to identify the sequences that act as transcriptional enhancers in a direct, quantitative, and genome-wide manner. For this, you can use the file edgeR_count. Quality Control¶. Kelley, Harold Pimentel, Steven L Salzberg , John L. To identify the genes that are involved in this fast pace of bone growth, an in vitro RNA-seq model that paralleled the sharp differences in bone growth between deer antlers and humans was established. While RNA-seq is becoming a de facto standard for monitoring the population of expressed transcripts in a given condition at a specific time, processing the huge amount of data it generates requires dedicated bioinformatics programs. RNA-seq (1) Peter N. The RNA-Seq read mapper TopHat produces output in this format, and is recommended for use with Cufflinks. Cufflinks will not normalize fragment counts by transcript length at all. py parse with gene annotation file (via -r) and reference genome sequence file (via -g). rna-seq experiments generate very large, complex data sets that demand fast, accurate and flexible software to reduce the raw read data to comprehensible results. RESULTS: To align our large (>80 billon reads) ENCODE Transcriptome RNA-seq dataset, we developed the Spliced Transcripts Alignment to a Reference (STAR) software based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching. for small RNA libraries, where no fragmentation takes place, or 3 prime end sequencing, where sampled RNA fragments are all essentially the same length). Introduction to RNA-seq Data Analysis Myrto Kostadima Romina Petersen < [email protected] FPKM is not a method of normalization. The software enables visualization of its output through probabilistically-weighted read alignments and read depth plots. For a super bare-bones picture of what gene expression is, please enjoy this ASCII art I made to illustrate the process:. RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. You will receive an email whenever this article is corrected, updated, or cited in the literature. , 2014) from whole kernel and whole endosperm material showed that nearly all endosperm-correlated modules (M10, M12, M15, M17, and M18) showed. RNA-Seq: Can Tophat/Cufflinks do anything that STAR cannot do? What is the best method to interpret TCGA, RNA sequence data? Bioinformatics: Why don't people publicly post the readcount (or aligned. Leave a comment RNA Isolation – Sea Star Coelomocytes (provided by Colleen Burge). 8 (for RNA alternative splicing analysis) and Cufflinks (for differential expression evaluation). Cufflinks assembles individual transcripts from RNA-seq reads that have been aligned to the genome. Conesa et al. Cufflinks is a tool for transcript assembly and expression analysis based on RNA-Seq data. I have 2x75b TruSeq stranded RNA Seq data from rat samples and collected on an Illumina NextSeq machine. Most current small RNA library preparation kits follow the RNA ligation method. CLC Genomics Workbench is a Desktop application for NGS analysis developed by CLCbio (www. bam I want. Lecture 1: Reference genome guided analysis. RNA-Seq has emerged as the standard for measuring gene expression and is an important technique often used in studies of human disease. cummeRbund is a visualization package for Cufflinks high-throughput sequencing data. In this step user supplies the genome les generated in the 1st step, as well as the RNA-seq reads (sequences) in the form of FASTA or FASTQ les. Later I did my Ph. 2019 2/15 動画とbiocondaによる install追加 STARは高速なRNAのアライメントツール。intron-exonのsplit-alingmentに対応している。動作はbowtie2より10倍以上高速とされ、マッピング感度の高さとエラー率の低さは既存のツールと同等とされている。. Long RNA-seq data standards have moved to https://www. RNA-seq Data Analysis Qi Sun, Robert Bukowski, Minghui Wang Bioinformatics Facility. In order to assist researchers in the RNA-seq field to deal with data analysis challenges, we implemented the RNA-seq web portal with three integrated workflows, which can be used for end-to-end RNA-seq data compute and analysis. STARR-seq (also known as self-transcribing active regulatory region sequencing) is a novel method to assay enhancer activity for millions of candidates from arbitrary sources of DNA. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. VIPER (Visualization Pipeline for RNA-seq analysis) is an analysis workflow that combines some of the most popular tools to take RNA-seq analysis from raw sequencing data, through alignment and quality control, into downstream differential expression and pathway analysis. Tuxedo Suite. These aligners are evaluated in terms of alignment yield, mismatches, base wise accuracy, splice junction detection and mapping errors. RNA-seq analysis has many different protocols; in each step of the following pipeline, alternative software tools can be chosen. rna-seq experiments generate very large, complex data sets that demand fast, accurate and flexible software to reduce the raw read data to comprehensible results. Performs gene and isoform level quantification from RNA-Seq data. The Cufflinks suite of tools can be used to perform a number of different types of analyses for RNA-Seq experiments. Single-cell RNA-seq assembly. , alternative splicing, allele-specific expression) one of the most important uses has. Hot Topics: RNA-seq Analysis in Galaxy. STAR mapping with Snakemake can save you a lot of time. single-cell-rna-seq free download. The evolution of RNA-seq analysis space is interesting. We detected 13,692 known transcripts and 3,724 previously unannotated ones, 62% of which are supported by independent expression data or by homologous genes in other species. the one who prepared the RNAseq library) which protocol they used to generate the data. In Cufflinks , an overlap graph is defined over the provided RNA sequence fragments, each represented as a node, in which two fragments are connected by an edge if they are compatible. RNA-seqlopedia is written by the Cresko Lab of the University of Oregon and was funded by grant R24 RR032670 (NIH, National Center for Research Resources). • Normalization of RNA-seq data in the context of differential analysis is essential in order to account for the presence of systematic variation between samples as well as differences in library composition. Cufflinks assembles transcripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples. Performs gene and isoform level quantification from RNA-Seq data. This video is about STAR. Compiled with gcc-5. performed a comprehensive analysis utilizing Ovation RNA-Seq System V2, Ovation Rapid Library System, Ovation Ultralow Library System, and Ovation Ultralow Methyl-Seq Library System to understand how epigenetics influences transcriptional regulation and chromosomal modifications. Several individual studies have compared the performance of STAR and other popular RNA-seq aligners, including GSMap, GEM, and TopHat, with experimental data and simulation data. •Compared to arrays, RNA-Seq is a very clean technical measure of expression –Generally don’t run technical replicates –Must run biological replicates •For clean systems (eg cell lines) 3x3 or 4x4 is common •Higher numbers required as the system gets more variable •Always plan for at least one sample to fail •Randomise across. It can handle designs involving two or more conditions of a single biological factor with or without a blocking factor (such as a batch effect or a sample pairing). 2019) SENSE Total RNA-Seq is a strand-specific library prep kit for accurate gene expression profiling, whole transcriptome sequencing, discovery, and quantification of antisense transcripts and overlapping genes. quantification using RNA-seq. Cufflinks was developed by Lior Pachter at UC Berkeley, Steven Salzberg’s computational genomics group at the Institute of Genetic Medicine at Johns Hopkins University, and Barbara Wold’s lab at Caltech. STARR-seq (also known as self-transcribing active regulatory region sequencing) is a novel method to assay enhancer activity for millions of candidates from arbitrary sources of DNA. RNA-Seq Tutorials • Tutorial 1 - RNA-Seq experiment design and analysis - Instruction on individual software will be provided in other tutorials • Tutorial 2 - Advanced RNA-Seq Analysis topics • Hands-on tutorials - Analyzing human and potato RNA-Seq data using Tophat and Cufflinks in Galaxy. Note that the knowledge that reverse transcriptase adds terminal Cs as it dissociates from template RNA is important. The similarity in microarray concordance kind of reminds me of Figure 2a from Rapport et al. Cufflinks (Trapnell et al 2010) is a popular software package for assembly and quantification of transcript using RNA-seq data. Q: Comparied with other circular RNA identification tools, what is the performance of CIRCexplorer2? A: CIRCexplorer2 is based on our previous circular RNA identification tool CIRCexplorer, and it inherits all the advantages of CIRCexplorer such as high accuracy, good sensitivity and low memory consumption. STAR alignment algorithm can be controlled by many user-defined parameters. Recent advances in RNA-Seq include single cell sequencing and in situ sequencing of fixed tissue. It typically ranges from 80 to 300 bp for RNA-seq. , 2008) or tilling arrays in the identification of previously unknown transcribed regions. •Opposite sequencing direction (“bridge sequencing”) •Distance < 400k bp •Example. BaseSpace TopHat Alignment App Maps reads, performs abundance estimations of reference genes and transcripts, calls variants, and offers optional fusion calling. CT-Star RNA Seq Full Workflow Integrated Service -- From sample to meaningful results, your portal to discover biology. 0f 2019/03/28. And so the Cufflinks tool will use the gene models from that existing annotation but align our RNA-seq data to it for estimating levels of expression and such. HISAT is a fast and sensitive spliced alignment program for mapping RNA-seq reads. RNA sequencing is performed on all HipSci iPS cell lines that are selected for banking after passing QC. Cufflinks will not normalize fragment counts by transcript length at all. Even at read-depths beyond 100 million, we find thousands of novel junctions,. Use this option when fragment count is independent of the size of the features being quantified (e. Together, they allow biologists to identify new genes and new splice variants of known ones, as well as compare gene and transcript expression under two or more conditions. •Compared to arrays, RNA-Seq is a very clean technical measure of expression –Generally don’t run technical replicates –Must run biological replicates •For clean systems (eg cell lines) 3x3 or 4x4 is common •Higher numbers required as the system gets more variable •Always plan for at least one sample to fail •Randomise across. Sep 12, 2013 • ericminikel. Cufflinks Detects Novel and Known Transcripts “To test Cufflinks, we sequenced and analyzed >430 million paired 75-bp RNA-Seq reads from a mouse myoblast cell line over a differentiation time series. It accepts aligned RNA-seq reads and assembles the alignments into a parsimonious set of transcripts. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. I have BAM files generated by STAR alignment and subsequently, I used this file as input to cufflinks. And there is no requirement that a reference genome must exist. Zylicz, et al. She describes the Cufflinks tool which is a spliced aligner. STAR mapping with Snakemake can save you a lot of time. TopHat is in a low maintenance, low support stage. The output files from the alignment are in SAM format. Galaxy provides the tools necessary to creating and executing a complete RNA-seq analysis pipeline. Cuffdiff Configuration. RNA-seq Data Analyses: In this module different bioinformatics tools for RNA-seq alignment will be described and tested. Align RNA-Seq reads using TopHat (splice-aware aligner). RNA-seq We offer the RNA-seq workflow assembles transcripts, estimates their abundances, compare your assembled transcripts to a reference annotation s, and show them using Genome browser. RNA sequencing. I have removed adapters from the FASTQ files and quality trimmed them using trimmomatic. RNA-Seq; DNA-Seq; RNA-Seq (Star) Pipeline Overview. Analysis of RNA-Seq Data Using TopHat and Cufflinks Posted by: RNA-Seq Blog in Analysis Pipelines November 4, 2015 8,155 Views The recent advances in high throughput RNA sequencing (RNA-Seq) have generated huge amounts of data in a very short span of time for a single sample. Tuxedo Suite. It is very popular to allow one mismatch for example. Hello, The RNA-seq reads must be aligned against a reference genome or transcriptome for use with Tophat. cufflinks -p 12 --library-type fr-firststrand \ Aligned. Allows users to handle RNA-sequencing pipeline based on the TopHat, Cufflinks and CummeRbund suite of software. This video is about STAR. Once you've obtained your single-cell RNA-seq data, the first thing you need to do with it is check the quality of the reads you have sequenced. Hi all, I am trying to determine differential expression between two test conditions - in rat. The mRNA Analysis pipeline begins with the Alignment Workflow, which is performed using a two-pass method with STAR. RNA sequencing (RNA-seq), a next-generation sequencing technique for transcriptome profiling, is being increasingly used, in part driven by the decreasing cost of sequencing. Cufflinks (Version in GenePattern public repository: 2. A large number of tools have been developed to tackle this problem, and we performed a comprehensive evaluation of these tools using both experimental. Cufflinks Input. Basic RNA-seq processing: unix tools and IGV RNA-seq (transcriptome sequencing) is a very powerful method for transcriptomic studies, that enables quantification of transcript levels as well as discovery of novel transcripts and transcript isoforms. Familiarity with Galaxy and the general concepts of RNA-seq analysis are useful for understanding this exercise. Salmon uses new algorithms (specifically, coupling the concept of quasi-mapping with a two-phase inference procedure) to provide accurate expression estimates very quickly (i. ENCORI Pan-Cancer Analysis Platform is designed for decoding Pan-Cancer Networks of lncRNAs, miRNAs, pseudogenes, snoRNAs, RNA-binding proteins (RBPs) and all protein-coding genes by analysing their expression profiles across 32 cancer types ( ~10,000 RNA-seq and ~9,900 miRNA-seq samples) integrated from TCGA project. The Cufflinks suite includes a number of different programs that work together to perform these analyses. We tested 12 different RNA-Seq quantification workflows: alignment with STAR or TopHat , followed by quantification by htseq-count with each of three options: union, intersection_strict and intersection_empty; alignment with STAR or TopHat followed by quantification with Cufflinks , both with and without multi-read correction (−−multi-read. In this lecture, I'll introduce STAR, an ultra fast RNA-seq aligner. The complete workflow, performing all the types of analyses Cufflinks can execute,. Christodoulou DC, et al. For example, the ENCODE project is currently using RNA-seq to characterize the transcriptome of the project's selection of cell lines [ 1 ]. In the Input Reference Sequences screen: Press the Add button. For more details on the SAM format, see the specification. RNA-seq is a very active field with many great analysis tools. A number of scRNA-seq protocols have been developed, and these methods possess their unique features with distinct advantages and disadvantages. BaseSpace TopHat Alignment App Maps reads, performs abundance estimations of reference genes and transcripts, calls variants, and offers optional fusion calling. RSEM is a software package that quantifies gene and isoform abundances from single-end (SE) or paired-end (PE) RNA-Seq data. RNA seq Analysis with STAR - Duration: 13:54. Nature protocols, 7(3), 562. Cufflinks Ψ estimates were inferred from transcript-level expression estimates. Back in 2015, our group described DEE, a user friendly repository of uniformly processed RNA-seq data, which I covered in detail in a previous post. The Cufflinks suite of tools can be used to perform a number of different types of analyses for RNA-Seq experiments. High-throughput RNA sequencing is an increasingly accessible method for studying gene structure and activity on a genome-wide scale. RNA-seq analysis, map with tophat2 or STAR? set flags so that the STAR output can be used with cufflinks. These lectures also cover UNIX/Linux commands and some programming elements of R, a popular freely available statistical software. RSEM (RNA-Seq by Expectation-Maximization) Updates. encodeproject. Their findings identify two enzymes (EZH2 and G9a. Alternate Protocol 8: Mapping RNA-seq reads and running Cufflinks to assemble and quantify transcripts for stranded RNA-seq data. Analysis of RNA-Seq Data Using TopHat and Cufflinks Posted by: RNA-Seq Blog in Analysis Pipelines November 4, 2015 8,155 Views The recent advances in high throughput RNA sequencing (RNA-Seq) have generated huge amounts of data in a very short span of time for a single sample. TopHat is designed to align RNA-seq reads to a reference genome, while Cufflinks assembles these mapped reads into possible transcripts and then generates a final transcriptome assembly. Familiarity with Galaxy and the general concepts of RNA-seq analysis are useful for understanding this exercise. DNase I was used to degrade any possible DNA contamination. STARsolo: mapping, demultiplexing and gene quantification for single cell RNA-seq. For RNA-Seq single-end sequencing for 50 base pair reads the RNA was isolated using Trizol reagent including a DNase I (Roche, Mannheim, Germany) digestion step. 2012, 7(3):562-78 Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. This is consistent with Cufflinks assembling. cummeRbund is a visualization package for Cufflinks high-throughput sequencing data. FastQC can be run in one of two modes. The Cufflinks suite includes a number of different programs that work together to perform these analyses. Children’s Research Institute, UTSW. RNA seq programs included are TopHat, CuffLinks, CuffDiff, CuffMerge, FastQC, and trimming using the FastX toolkit. Ours was the first such repository that wasn't limited to human or mouse and included sequencing data from a variety of instruments and library types. Obtain a new transcriptome using CuffLinks & CuffM. Another RNA-Seq study showed altered expression of gene transcripts involved in neuroplasticity, such as PROM1, ABCG2, and FLI1, and circadian rhythms, such as OSBPL3, GANAB, SRSF5, and RFX4, in the dorsolateral prefrontal cortex of individuals with BD. The MinION Oxford Nanopore sequencer allows sequencing long reads, with the potential of sequencing complete genes, and even complete genomes of viruses, in individual reads. Aligns RNA-Seq reads with the STAR aligner and assigns aligned reads to genes, followed by differential expression with DESeq2. I am using Cygwin software in order to create a Linux environment on my windows machine. cummeRbund is a visualization package for Cufflinks high-throughput sequencing data. It contains over 450 analysis tools and a large collection of reference genomes. The protocol covers read alignment with TopHat, gene and transcript discovery with Cufflinks, annotation analysis with Cuffmerge and Cuffcompare. We’ve looked at the RNA data with a basic STAR-HTSeq-edgeR pipeline, then plugged it into Pathview for a look at some pathway analysis. RNA-seq analysis, map with tophat2 or STAR? set flags so that the STAR output can be used with cufflinks. toTranscriptome. For more details on the SAM format, see the specification. Familiarity with Galaxy and the general concepts of RNA-seq analysis are useful for understanding this exercise. We will first run Cufflinks on the Tophat condition 1 results. Cufflinks is an integrated transcriptome analysis tool from transcript assembles, quantification, and comparison between different conditions. RNA-Seq (Star) Pipeline Overview¶. That said, our primary purpose is to provide guidance on setting up batch submission scripts through PBS, and how much computing resource one needs to request for analyzing a human transcriptome data set. Participants will explore experimental design, cost estimation, data generation, and analysis of RNA-Seq data generated on the Illumina sequencing platform. CT-Star RNA Seq Full Workflow Integrated Service -- From sample to meaningful results, your portal to discover biology. RNA-Seq Data Analysis Course - NGS Workshop 2016 Toggle navigation. This is consistent with Cufflinks assembling. Decoding cancer transcriptomes using public RNA-seq data dr. using STAR+Cufflinks for transcript assembly turns unstranded RNA-seq to stranded? I am trying to use STAR+Cufflinks to do a reference based transcript assembly using unstranded RNA-seq data. Differential Expression Analysis of RNA Sequencing Data using Cufflinks. 0 project conducted deep sequencing for total RNA of 16 individual human tissues with one sample for each tissue type. Nevertheless, the analysis of the massive amounts of data generated by large-scale RNA-seq remains a challenge. It accepts aligned RNA-Seq reads and assembles the alignments into a parsimonious set of transcripts. Following standard pre-processing steps, reads were mapped to the hg38 human genome using STAR, resulting in an. There are often numerous mapping parameters that we can specify, but usually the default mapping parameters are fine. Over the last few years, single-cell RNA-sequencing (scRNAseq) has emerged as a revolutionary tool with the potential to explore biological heterogeneity at the most basic level of organismal organization. Cufflinks (Trapnell et al 2010) is a popular software package for assembly and quantification of transcript using RNA-seq data. RNA-Seq workflow is divided into several steps: RNA extraction, library construction, sequencing, and data analysis. gene expression levels) from a small sample set (e. One of the main challenge in single-cell (sc)RNA-seq is the barcoding (or indexing) step. bam I want. RNA-Seq has replaced microarrays for many applications in the area of biomarker discovery. The RNA-Seq read mapper TopHat produces output in this format, and is recommended for use with Cufflinks. Cufflinks takes a text file of SAM alignments, or a binary SAM (BAM) file as input. See the lecture notes and lab. 0, and is shown on the right. In particular, TopHat and Cufflinks require a sequenced genome (see below for references to tools that can be used without a reference genome). TopHat and Cufflinks are free, open-source software tools for gene discovery and comprehensive expression analysis of high-throughput mRNA sequencing (RNA-seq) data. Tuxedo is a program that enables assessment of alternative splicing (AS) inferred on fragments per kilobase per million (FPKM) values. In recent years single cell RNA-seq (scRNA-seq) has become widely used for transcriptome analysis in many areas of biology. In a major advance for genomics, it is now possible to look at what one single cell is doing at any given time with a technique called single-cell RNA sequencing (scRNA-seq). DressUp facilitates RNA seq programs by streamlining various packages into a single script. Re-process from FASTQ Benchmark to find out if we can obtain a sensible clustering of samples from different sources. NOTE: If you want to learn about. rna-seq experiments generate very large, complex data sets that demand fast, accurate and flexible software to reduce the raw read data to comprehensible results. HISAT is a fast and sensitive spliced alignment program for mapping RNA-seq reads. RNA-Seq is a revolutionary tool, based in next-generation sequencing (NGS) technologies, to obtain the profiling of the full transcriptome of any organism (Wang, Gerstein, & Snyder, 2009). Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. The Illumina ligation method is a well-know example of this class and is based on sequential ligation of two different adapters. 2019) SENSE Total RNA-Seq is a strand-specific library prep kit for accurate gene expression profiling, whole transcriptome sequencing, discovery, and quantification of antisense transcripts and overlapping genes. Cufflinks (Trapnell et al 2010) is a popular software package for assembly and quantification of transcript using RNA-seq data. 3 of 14 RNA-Seq Differential Expression using Cufflinks Suite Next-Generation Sequencing requires new algorithms to process the large quantity of data produced. RNA-Seq Tutorials • Tutorial 1 – RNA-Seq experiment design and analysis – Instruction on individual software will be provided in other tutorials • Tutorial 2 – Hands-on using TopHat and Cufflinks in Galaxy • Tutorial 3 – Advanced RNA-Seq Analysis topics. The Direct RNA Sequencing kit (SQK-RNA002) is used to prepare any RNA with a 3’ polyA tail for 1D sequencing on the Oxford Nanopore sequencing devices. The pre-compiled STAR executables are located bin/ subdirectory. RNA-seq Data Analyses: In this module different bioinformatics tools for RNA-seq alignment will be described and tested. Over the past five years, RNA-sequencing (RNA-seq) has been gradually overtaking microarrays to become the tool of choice for genome-wide analysis of the transcriptome. Cufflinks takes a text file of SAM alignments, or a binary SAM (BAM) file as input. I have removed adapters from the FASTQ files and quality trimmed them using trimmomatic. CummeRbund is a collaborative effort between the Computational Biology group led by Manolis Kellis at MIT's Computer Science and Artificial Intelligence Laboratory,. We have RNA-Seq data (HiSeq) and proteomic data (iTRAQ). RNA-Seq STAR mapping with Snakemake. 7 samples were taken before and 7 after androgen ablation therapy treatment. Sample 2 as well as Gene A vs. The advantage of STAR is that time of alignment can be shorten into few hours or even in an hour with similar accuracy of alignment as TopHat. The Cufflinks RNA-Seq workflow. We applied this pipeline to RNA-seq analysis of single cells isolated from embryonic mouse lung at E16. RNA-Seq is a revolutionary tool, based in next-generation sequencing (NGS) technologies, to obtain the profiling of the full transcriptome of any organism (Wang, Gerstein, & Snyder, 2009). RNA sequencing. Quality Control; Genome Alignment using Star; Gene Expression Quantification using Cufflink; Result Explained. Includes the data used in the analysis, so you can try it yourself and explore further. Supporting a broad range of applications, including gene expression profiling, chromosome counting, detection of epigenetic changes, and molecular analysis, NGS is. This is a course designed to give you an overview of RNA-Sequencing in a hands-on manner. In contrast, alignment approaches preserves the information in the reads, but the alignment process is slower. Results We benchmarked STAR-Fusion’s fusion detection accuracy using both simulated and genuine Illumina paired-end RNA-Seq data, and show that it has superior performance compared to popular alternative fusion detection. A large number of tools have been developed to tackle this problem, and we performed a comprehensive evaluation of these tools using both experimental. Hi all, I have a stranded (fr-firststrand) paired-end RNA-seq data. Cufflinks appears worse in this respect, as on average it assigns the equivalent of 81% of mean TP expression to FP transcripts, while for Oases this value is only 41% (Table 2). Splice variant quantification with SGSeq, MISO and Cufflinks for real RNA-seq data. Cuffdiff requires just a few inputs. In the Input Reference Sequences screen: Press the Add button. I’ve had STAR require > 32 GiB of memory for the human genome, with a moderate number of threads. An in-house RNA-Seq dataset was used and six types of results were generated. Galaxy provides the tools necessary to creating and executing a complete RNA-seq analysis pipeline. While RNA-seq is becoming a de facto standard for monitoring the population of expressed transcripts in a given condition at a specific time, processing the huge amount of data it generates requires dedicated bioinformatics programs. In contrast to our previous Tang2009 protocol that employed oligo(dT) 24 primers to convert the poly(A)+ mRNAs into cDNAs, SUPeR-seq uses random (AnchorX-T 15 N 6) primers to enable the simultaneous detection of both poly(A)+ and poly(A)- RNA species from a single cell (Fig. I did mapping with tophat and STAR. Supporting a broad range of applications, including gene expression profiling, chromosome counting, detection of epigenetic changes, and molecular analysis, NGS is.
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Capitol Hill Just Passed a #GoodDealforWomen Through Tax Reform December 19 2017 by Patrice Lee Onwuka
California’s $15 Minimum Wage to Cost 400,000 Jobs – Many for Women December 18 2017 by Patrice Lee Onwuka
Pew: Gender Discrimination, Sexual Harassment are Problems but Not Universal December 15 2017 by Patrice Lee Onwuka
Alyssa Milano, John Oliver Aren’t for Saving the Internet but Internet Control December 14 2017 by Patrice Lee Onwuka
Feminism is 2017’s Word of the Year But Women Still Don’t Know What It Means December 13 2017 by Patrice Lee Onwuka
San Francisco Embraces Everyone but Delivery Robots December 12 2017 by Patrice Lee Onwuka
Jobs Report: One Million More Women Are Working Since November 2016 December 8 2017 by Patrice Lee Onwuka
What Paris Hilton Could Teach Chelsea Handler About Grace in Crisis December 8 2017 by Patrice Lee Onwuka
Kellyanne Conway Had a #MeToo Moment Too, But No One Paid Attention Because of Politics December 7 2017 by Patrice Lee Onwuka
Mark Zuckerberg’s Paternity Leave Tells Us 3 Things About Paid Leave, Millennials December 6 2017 by Patrice Lee Onwuka
Sheryl Sandberg Calls Out the Chilling Effect Between Sexes Following Harassment Revelations December 5 2017 by Patrice Lee Onwuka
Three Ways to Maximize Your Last Paychecks of 2017 December 1 2017 by Patrice Lee Onwuka
Meghan Markle Giving Up Her Career for Prince Harry Doesn’t Make Her a Bad Woman November 29 2017 by Patrice Lee Onwuka
Susan Sarandon is Still Anti-Hillary, The Left Blasts Her For It November 28 2017 by Patrice Lee Onwuka
FCC’s Thanksgiving Blessing was to End Internet Neutrality Regulations November 27 2017 by Patrice Lee Onwuka
Black Friday Protests Dwindle Exposing This Uncomfortable Truth November 27 2017 by Patrice Lee Onwuka
Kathy Griffin’s Sadistic Trump Joke Left Her Jobless, She’s Not a Victim November 21 2017 by Patrice Lee Onwuka
New York Wants to Dictate When You Work November 20 2017 by Patrice Lee Onwuka
Bernie Sanders Blatantly Gives Bill Clinton, Al Franken a Pass on Sexual Harassment November 20 2017 by Patrice Lee Onwuka
Do Winter Blizzards Call for Paid Leave Too? November 15 2017 by Patrice Lee Onwuka
‘The Simpsons’ Calling Kellyanne Conway a Nazi is a Sexist, Racist Cheap Shot November 14 2017 by Patrice Lee Onwuka
How Zero Tolerance Polices Backfire in the Age of #MeToo November 13 2017 by Patrice Lee Onwuka
Three G.I. Janes from American History that Make Women Proud on Veterans Day November 10 2017 by Patrice Lee Onwuka
TSA Failing Undercover Weapons Tests – Again, and It’s Disturbing November 10 2017 by Patrice Lee Onwuka
NAACP Exploits NFL Protests to Ban "Star-Spangled Banner" as National Anthem November 9 2017 by Patrice Lee Onwuka
Women in Congress Have an Answer to Paid Leave November 8 2017 by Patrice Lee Onwuka
Sexist LA Times Columnist Attacks Sarah Huckabee Sanders’ Looks November 6 2017 by Patrice Lee Onwuka
Explosive: DNC, Hillary Clinton Campaign Rigged the Party Nomination for Her November 3 2017 by Patrice Lee Onwuka
Eva Longoria is Desperately Wrong to Push the Latina Pay Gap November 2 2017 by Patrice Lee Onwuka
Shame on Cosmo for Normalizing Incest – Again November 2 2017 by Patrice Lee Onwuka
Poll: Most Women Love Tech but Only Half Think It’ll Improve Their Life November 1 2017 by Patrice Lee Onwuka
2018 Obamacare Premiums to Leave Even Wider Hole in Your Wallet October 30 2017 by Patrice Lee Onwuka
Walmart Debuts Robots to Make (Holiday) Shopping Better October 30 2017 by Patrice Lee Onwuka
NAACP Issues Inane Flying-While-Black Advisory, Please Keep It to Yourselves October 27 2017 by Patrice Lee Onwuka
President Trump’s Opioid Attack Plan: Personal, Practical, and Policy October 26 2017 by Patrice Lee Onwuka
UPenn Teacher Helps Black Women by Discriminating Against White Men October 24 2017 by Patrice Lee Onwuka
Campus Sexual Assault: The Distressing Fight Mothers Face for Their Sons, Advice to Young Women October 23 2017 by Patrice Lee Onwuka
IRS to Hold Tax Returns Hostage to Enforce Obamacare October 20 2017 by Patrice Lee Onwuka
ABC’s Misleading Workplace Sexual Harassment Poll Pushes an Agenda October 19 2017 by Patrice Lee Onwuka
‘The Big Bang Theory’ Actress Counsels Actresses to be Modest, Triggers Feminist Backlash October 17 2017 by Patrice Lee Onwuka
No, NFL Protests Aren't About the Gender Pay Gap October 16 2017 by Patrice Lee Onwuka
Ivanka Trump to Boost Global Women Entrepreneurs Via World Bank Fund October 16 2017 by Patrice Lee Onwuka
President Trump’s Anti-Regulation Crusade may be Working in Americans’ Eyes October 13 2017 by Patrice Lee Onwuka
Lawmakers Try to Stop Secretary DeVos on Campus Sexual Assault Policy October 13 2017 by Patrice Lee Onwuka
Black Lives Matter Group Opposes Free Speech for Every American October 12 2017 by Patrice Lee Onwuka
Harvey Weinstein’s Open Secret Exposes Hypocrisy on the Left October 10 2017 by Patrice Lee Onwuka
Marc Jacobs Doubles Down on Free Expression for Designers, Artists October 10 2017 by Patrice Lee Onwuka
On Columbus Day, Give Him a Break October 9 2017 by Patrice Lee Onwuka
Company Behind Fearless Girl Statute Admits Gender Pay Issues October 9 2017 by Patrice Lee Onwuka
Three Bright Spots for Women in Today’s Jobs Report October 6 2017 by Patrice Lee Onwuka
Half of Americans Can’t Afford More than $100 in Healthcare Premiums October 4 2017 by Patrice Lee Onwuka
One Tech Company Changed Approach to Promotions, Women Won Big October 3 2017 by Patrice Lee Onwuka
Librarian Rejects Dr. Seuss Books Because Melania Trump Sent Them September 29 2017 by Patrice Lee Onwuka
Amber Rose’s Slutwalk is a Stunt Not Empowerment September 29 2017 by Patrice Lee Onwuka
Do We Want a Feminist ‘Will & Grace’ Reboot? September 26 2017 by Patrice Lee Onwuka
Hillary Clinton Slams Women Who Support Trump as “Publicly Disrespecting Themselves” September 26 2017 by Patrice Lee Onwuka
Betsy DeVos Ends Obama-Era Policies on School Sexual Assault September 22 2017 by Patrice Lee Onwuka
Washington Wants Black Households to Trade Privacy for Cash September 22 2017 by Patrice Lee Onwuka
Half of College Women Get This Wrong about Hate Speech September 20 2017 by Patrice Lee Onwuka
Joe Biden Slams Socialist Universal Basic Income Idea September 19 2017 by Patrice Lee Onwuka
Millennials Would Trade Right to Vote for Student Loan Freedom September 18 2017 by Patrice Lee Onwuka
Feminists Attack Starbucks's Pumpkin Spice Lattes for Funding White Supremacy September 15 2017 by Patrice Lee Onwuka
DeVos Backlash Incites Dangerous Language September 14 2017 by Patrice Lee Onwuka
Over One in Three Americans Can’t Name Any 1st Amendment Rights September 12 2017 by Patrice Lee Onwuka
Miss America Pageant Gets Political and Blatantly Anti-Trump September 11 2017 by Patrice Lee Onwuka
Melania Trump Staffs Fewer People than Michelle Obama – A Savings for Taxpayers September 8 2017 by Patrice Lee Onwuka
Hillary Clinton Asks 'What Happened?' in 2016 September 7 2017 by Patrice Lee Onwuka
Kim Kardashian Still Eschews One Label: Feminist September 5 2017 by Patrice Lee Onwuka
Jerry Springer Rides the $15 Minimum Wage Wagon September 5 2017 by Patrice Lee Onwuka
A $20 Question: Will Harriet Tubman be on the Bill? September 1 2017 by Patrice Lee Onwuka
Estee Lauder Faces Heat Over Paid Leave for Dads August 31 2017 by Patrice Lee Onwuka
No, Melania’s Shoes are Not the Story, Texas is August 30 2017 by Patrice Lee Onwuka
Illinois Governor Shuts Down $15 Minimum Wage August 28 2017 by Patrice Lee Onwuka
Amy Schumer's Equal Pay Problem August 25 2017 by Patrice Lee Onwuka
Poll: Nearly Three Out of Four Americans Would Die to Defend Freedom of Speech August 25 2017 by Patrice Lee Onwuka
Here’s Another Way Women Suffer from Minimum Wage Increases August 24 2017 by Patrice Lee Onwuka
Oops! Social Security Website Misdirects $11 Mil to Thieves August 23 2017 by Patrice Lee Onwuka
D.C. Raises Minimum Wage, Diners Foot the Bill August 21 2017 by Patrice Lee Onwuka
What Dr. Ben Carson Shows Us about Confronting Hateful Ideas August 17 2017 by Patrice Lee Onwuka
Hope Brings Another Woman to Trump Leadership Circle August 17 2017 by Patrice Lee Onwuka
Helen Mirren’s Feminism is about Anti-Trump and Potty-Mouths August 16 2017 by Patrice Lee Onwuka
CFPB Crackdown May Trap Borrowers in a Different Debt Trap August 14 2017 by Patrice Lee Onwuka
Sheryl Sandberg Leans In to One-Size-Fits-All Paid Leave Plan August 10 2017 by Patrice Lee Onwuka
Café Charges 18% Man Tax to Close the Gender Pay Gap August 9 2017 by Patrice Lee Onwuka
College STEM Women Claim to be Sexism Victims August 7 2017 by Patrice Lee Onwuka
A Sexism Rating Coming to a Movie Near You August 3 2017 by Patrice Lee Onwuka
IRS Rehires Hundreds of Fired Workers Despite Serious Transgressions August 3 2017 by Patrice Lee Onwuka
Study: MD County to Lose 47,000 jobs because of $15 Minimum Wage August 2 2017 by Patrice Lee Onwuka
Adam Carolla Schools Congress on Campus Free Speech July 31 2017 by Patrice Lee Onwuka
How One Tech Company is Taking on the Future of Work July 28 2017 by Patrice Lee Onwuka
Governor Christie Shuts Down Costly Paid Leave Expansion Bill July 27 2017 by Patrice Lee Onwuka
Teachers’ Union Boss Plays the Race Card against Secretary DeVos July 25 2017 by Patrice Lee Onwuka
Cutting the Strings on Obamacare Enrollment Assistance July 24 2017 by Patrice Lee Onwuka
Not Even Death Stops a Government Check July 21 2017 by Patrice Lee Onwuka
Who Cares for the Caregivers? July 19 2017 by Patrice Lee Onwuka
College Diversity Officials Rake in Major Dough July 18 2017 by Patrice Lee Onwuka
DHS Hides Spending $3.6 Mil of Taxpayer Dollars on Pricey Conferences July 17 2017 by Patrice Lee Onwuka
Study: Marrying Before Having Kids is a Strong Path to Success for Millennials July 13 2017 by Patrice Lee Onwuka
$2 Mil NJ Welfare Fraud Exposes Holes in Our Social Safety Net July 7 2017 by Patrice Lee Onwuka
“WTF” is Silicon Valley’s Next Answer to the Democratic Party July 6 2017 by Patrice Lee Onwuka
Fraud Alert: Federal Free Phone, Internet Program Wastes $100 Mil Annually on Fake, Dead Enrollees July 3 2017 by Patrice Lee Onwuka
Cosmo Leaves Conservatives Off Their Short List of Female Presidential Candidates June 28 2017 by Patrice Lee Onwuka
Report: Seattle’s Minimum Wage Hike Gave Low-Wage Workers a $125 Monthly Pay Cut June 27 2017 by Patrice Lee Onwuka
Obama Comes Out to Bash Senate Repeal Bill June 23 2017 by Patrice Lee Onwuka
Maybe Profit Motivates Tech Companies More than Politics June 22 2017 by Patrice Lee Onwuka
Ivanka Trump Hits Capitol Hill about Paid Leave June 20 2017 by Patrice Lee Onwuka
Supreme Court Upholds Offensive Names as Free Speech June 20 2017 by Patrice Lee Onwuka
Silicon Valley CEOs Ignore Calls to Boycott Trump Tech Summit June 19 2017 by Patrice Lee Onwuka
What the Return of Pro-Net Neutrality Democrat Means for Internet Regs Rollback June 15 2017 by Patrice Lee Onwuka
Is the Federal Consumer Cop Asleep on the Job? June 15 2017 by Patrice Lee Onwuka
Obama Health Records Program Leaves Taxpayers on the Hook for Mispayments June 15 2017 by Patrice Lee Onwuka
Just in Time for July 4th New York State Greenlights Uber, Lyft June 9 2017 by Patrice Lee Onwuka
Filter, Don’t Pilfer June 8 2017 by Patrice Lee Onwuka
What Michelle Obama Gets Wrong About Self-Interest June 8 2017 by Patrice Lee Onwuka
Harvard Revokes Acceptances Over Online Comments June 6 2017 by Patrice Lee Onwuka
Bill Maher’s N-Word Episode June 5 2017 by Patrice Lee Onwuka
Hillary Clinton's Whine Tour Stops in Silicon Valley June 2 2017 by Patrice Lee Onwuka
Maryland Governor Shuts Down Paid Sick-Leave Bill May 26 2017 by Patrice Lee Onwuka
Chelsea Clinton Equates Child Marriage to Climate Change May 26 2017 by Patrice Lee Onwuka
Obamacare’s Death Spiral Claims Another Healthcare Insurer May 25 2017 by Patrice Lee Onwuka
Top 10 Unauthorized Federal Programs on the Chopping Block in Trump Budget May 25 2017 by Patrice Lee Onwuka
Supreme Court Ends Judge Shopping for Patent Trolls May 23 2017 by Patrice Lee Onwuka
State of Texas Trumps Austin over Uber, Lyft Regulations May 22 2017 by Patrice Lee Onwuka
Undoing Net Neutrality Starts Today May 18 2017 by Patrice Lee Onwuka
San Francisco May Stall Delivery Robots May 18 2017 by Patrice Lee Onwuka
Miss USA Stands by Her Comments – Good for Her May 17 2017 by Patrice Lee Onwuka
Miss USA Slammed for Saying Healthcare is a Privilege Rather than a Right May 15 2017 by Patrice Lee Onwuka
Don’t Be Tone Deaf on Hearing Aids May 11 2017 by Patrice Lee Onwuka
Grads Boo Betsy DeVos at Commencement May 11 2017 by Patrice Lee Onwuka
TV Comedian John Oliver Piles on to Save Obama Internet Rules May 10 2017 by Patrice Lee Onwuka
Automation Coming to a City near You May 9 2017 by Patrice Lee Onwuka
Your Zip code Is a Good Predictor for How Long You Live May 9 2017 by Patrice Lee Onwuka
Tech Bosses: Expect Significant Automation of Jobs by 2022 May 5 2017 by Patrice Lee Onwuka
Can Another Tech Squad to Transform How Washington Works? May 5 2017 by Patrice Lee Onwuka
Tech Founder to Ivanka Trump: Don’t Use My Story May 3 2017 by Patrice Lee Onwuka
Are Saving and Debt Repayment the New Normal for Americans? May 2 2017 by Patrice Lee Onwuka
This Immigrant is Showing Up to Work on May Day May 1 2017 by Patrice Lee Onwuka
FCC Announces Net Neutrality Reversal Plan April 27 2017 by Patrice Lee Onwuka
Copyright Office Reform Bill Passes House April 27 2017 by Patrice Lee Onwuka
FCC Expected to Kick Net Neutrality to the Curb April 26 2017 by Patrice Lee Onwuka
Delivery Robots Get Legislative Green Light in Two States April 25 2017 by Patrice Lee Onwuka
Harvard Study: Higher Minimum Wages Trigger Restaurant Closures April 24 2017 by Patrice Lee Onwuka
Not Done Rolling Back Washington’s Red Tape Yet April 19 2017 by Patrice Lee Onwuka
Three Celebrities Feeling the Pain on Tax Day April 18 2017 by Patrice Lee Onwuka
Female-Led FTC Takes Reducing Regulations Seriously April 18 2017 by Patrice Lee Onwuka
New York Pilots Free College Tuition Plan April 13 2017 by Patrice Lee Onwuka
Poll: Americans Comfortable with AI in Most--But Not All--Instances April 11 2017 by Patrice Lee Onwuka
No Phone Calls on Airplanes After All April 11 2017 by Patrice Lee Onwuka
Amazon to Hire 30,000 Part-time, Work-from-Home Jobs April 10 2017 by Patrice Lee Onwuka
Judge Blocks Seattle’s Plan to Unionize Uber April 7 2017 by Patrice Lee Onwuka
Stop the Misinformation on the Roll-Back of Privacy Rules April 5 2017 by Patrice Lee Onwuka
The Challenge of Counterfeit Drugs April 4 2017 by Patrice Lee Onwuka
Cali Lawmaker Wants to Push Gig-Economy Workers into Unions April 3 2017 by Patrice Lee Onwuka
FCC Reverses Federal Power Grab Over LifeLine Program (AKA ObamaPhones for the Internet) March 30 2017 by Patrice Lee Onwuka
Here’s How Uber is Boosting Diverse Applicants March 30 2017 by Patrice Lee Onwuka
Congress Scraps Obama-Era Privacy Rules March 30 2017 by Patrice Lee Onwuka
Should We Worry About Robots Replacing Workers? March 27 2017 by Patrice Lee Onwuka
Black Leaders Tell Trump What They Have to Lose March 23 2017 by Patrice Lee Onwuka
Congress to Free the Copyright Office from the Thumb of the Library of Congress March 23 2017 by Patrice Lee Onwuka
Why Everything from Dresses to Avocados May Get More Expensive March 22 2017 by Patrice Lee Onwuka
Trump, Clinton Campaigns Depended Upon Sharing Economy on the Campaign Trail March 21 2017 by Patrice Lee Onwuka
Before You Blame the Wage Gap, Check Your Attitude March 20 2017 by Patrice Lee Onwuka
Trump Hacks Away at the TSA March 20 2017 by Patrice Lee Onwuka
Trump Pumps the Brakes on Obama-Era Fuel Efficiency Regs March 16 2017 by Patrice Lee Onwuka
Students Push Back on Feminist Professor’s Indoctrination March 15 2017 by Patrice Lee Onwuka
President Trump: May Be Time to Say "You're Fired!" to Duplicative Agency Employees March 14 2017 by Patrice Lee Onwuka
Austin’s Struggle with Transportation After Uber, Lyft Ride Out of Town March 13 2017 by Patrice Lee Onwuka
Steve Case: American Workers Feeling Left Behind, Really Have Been Left Behind March 13 2017 by Patrice Lee Onwuka
Analogies Fly Over Net Neutrality March 9 2017 by Patrice Lee Onwuka
These are the Women A Day Without Women Hurts March 8 2017 by Patrice Lee Onwuka
Can Facebook’s New Tool Crack Down on “Fake News”? March 7 2017 by Patrice Lee Onwuka
Is Regulating the Internet a Good Idea? March 6 2017 by Patrice Lee Onwuka
Dr. Ben Carson Confirmed as HUD Head March 3 2017 by Patrice Lee Onwuka
Robots Green-Lighted to Deliver Your Packages March 2 2017 by Patrice Lee Onwuka
Three Key Messages to Black America from Trump’s Address March 1 2017 by Patrice Lee Onwuka
Reports: GM Lobbies to Keep Competing Self-Driving Tech Off the Road February 28 2017 by Patrice Lee Onwuka
New Self-driving Truck Company Speeds Up the Technology February 27 2017 by Patrice Lee Onwuka
Small Internet Companies Gain Regulatory Relief from Washington February 24 2017 by Patrice Lee Onwuka
Women Have a New Ally in Getting Them to Work February 24 2017 by Patrice Lee Onwuka
Federal Watchdog: Obama’s Techie Squad Flouted the Rules February 22 2017 by Patrice Lee Onwuka
Adulting School: Millennials Are Signing Up for Classes February 22 2017 by Patrice Lee Onwuka
Former AG Eric Holder Leading Internal Investigation at Uber February 21 2017 by Patrice Lee Onwuka
Whoopi Snubs the Mean Girls at New York Fashion Week in Defense of Tiffany Trump February 17 2017 by Patrice Lee Onwuka
Humana Calls It Quits on ObamaCare, Leaving One State in Trouble February 16 2017 by Patrice Lee Onwuka
Congress Opens the Door to Self-Driving Cars February 15 2017 by Patrice Lee Onwuka
Linda McMahon Confirmed to Head the SBA February 14 2017 by Patrice Lee Onwuka
Cabs Threaten to Halt Wheelchair Access Over Uber, Lyft February 14 2017 by Patrice Lee Onwuka
Trump Models: The Next Target in the Fashion World Purge February 13 2017 by Patrice Lee Onwuka
Senate Dems Come Out Swinging to Protect Washington’s Control of the Internet February 8 2017 by Patrice Lee Onwuka
A Surprise Stand for Competition Over Cronyism in Battle for NYC Streets? February 8 2017 by Patrice Lee Onwuka
Lifeline Internet Assistance Program (AKA Obamaphones for the Internet) Takes a Hit February 7 2017 by Patrice Lee Onwuka
Big Mac ATM’s, the Answer to $15 Minimum Wages? February 2 2017 by Patrice Lee Onwuka
New FCC Chairman Gets to Work Cutting Red Tape February 2 2017 by Patrice Lee Onwuka
Trump Transportation Secretary Chao Confirmed, Despite Democratic Protest February 1 2017 by Patrice Lee Onwuka
Cutting the Red Tap: President Trump Orders 2-for 1 Rule on Regulations January 31 2017 by Patrice Lee Onwuka
Chelsea Handler's Garbage-Pail Mouth is Now Shaming Melania Trump's English January 27 2017 by Patrice Lee Onwuka
Break out the Dow 20K Hats? January 27 2017 by Patrice Lee Onwuka
Apple Hit with Another Lawsuit over Distracted Driving January 25 2017 by Patrice Lee Onwuka
Getting American Workers Ready for Automation’s Coming Shake-Up January 24 2017 by Patrice Lee Onwuka
Uber Ponies Up $20M to Feds Over Driver Pay January 23 2017 by Patrice Lee Onwuka
Americans Aren’t Really Warming Up to Self-Driving Cars January 19 2017 by Patrice Lee Onwuka
The Government’s “Slant” on Trademarks Gets Challenged January 19 2017 by Patrice Lee Onwuka
Uber Sues Seattle to Block Drivers from Unionizing January 18 2017 by Patrice Lee Onwuka
Inauguration Reminds Us Why Home Sharing is a Win for Travelers January 17 2017 by Patrice Lee Onwuka
Jesse Jackson PUSHes Uber on Diversity Statistics January 13 2017 by Patrice Lee Onwuka
5 Times President Obama’s Rhetoric Didn’t Match His Kumbaya Message January 11 2017 by Patrice Lee Onwuka
Jenna Bush Microaggressess at the Golden Globes? January 10 2017 by Patrice Lee Onwuka
Rolling Back Obama’s Internet Privacy Rules January 9 2017 by Patrice Lee Onwuka
Update: City of San Diego Says Restaurants Can't Publicly Blame Surcharge on Minimum Wage Hike January 6 2017 by Patrice Lee Onwuka
San Diego Restaurants Slap Diners with Surcharges Thanks to Minimum Wage Increases January 6 2017 by Patrice Lee Onwuka
The Uber Hook-Up Effect January 5 2017 by Patrice Lee Onwuka
Christmas Eve Distracted Driving Fatality Could Trigger Government Action January 3 2017 by Patrice Lee Onwuka
Cali Regulators Drive Uber’s Self-Driving Cars Away December 27 2016 by Patrice Lee Onwuka
Will Seattle’s Driver Unionization be the End of Uber, Ridesharing? December 21 2016 by Patrice Lee Onwuka
Not Fake News: Some UVA Students Sign Fake Anti-Christmas Petition December 20 2016 by Patrice Lee Onwuka
Pinterest Misses Female Recruitment Quantity Quotas, But Hits on Quality December 19 2016 by Patrice Lee Onwuka
No Michelle Obama, “Hope” Doesn’t Start and End with Your Husband December 19 2016 by Patrice Lee Onwuka
Uber, San Francisco Regulators Crash Head-on over Self-Driving December 16 2016 by Patrice Lee Onwuka
More on the Shaming of Wonder Woman December 15 2016 by Patrice Lee Onwuka
Environmental Group Smears Black Beauty Products December 14 2016 by Patrice Lee Onwuka
San Francisco Mayor Sides with Airbnb December 12 2016 by Patrice Lee Onwuka
Airbnb Can’t Beat ‘Em, So Now They’re Lobbying ‘Em December 9 2016 by Patrice Lee Onwuka
The Supremes Side with Samsung Against Apple in High-Profile Patent Case December 7 2016 by Patrice Lee Onwuka
Cell Phones on the Road: A Serious Problem, but Do the Feds Need to Solve It? December 6 2016 by Patrice Lee Onwuka
Airbnb Took on the Big Apple and Lost December 5 2016 by Patrice Lee Onwuka
Obama’s Overtime Rule is Down, but Not Out December 2 2016 by Patrice Lee Onwuka
Taxpayers Will Forgive Billions More in Student Loan Debt December 2 2016 by Patrice Lee Onwuka
Uber Drivers Join Minimum Wage Protesters November 30 2016 by Patrice Lee Onwuka
Negative Online Reviews Get New Federal Protection November 30 2016 by Patrice Lee Onwuka
#GivingTuesday: the Hangover Cure for a Weekend of Spending November 29 2016 by Patrice Lee Onwuka
Some Maine Restaurant Workers Worried about Effects of Minimum Wage Hike November 28 2016 by Patrice Lee Onwuka
Judge Blocks Obama Workplace Overtime Rules November 23 2016 by Patrice Lee Onwuka
Pew: One in Four Americans Makes Bank through the Gig Economy November 21 2016 by Patrice Lee Onwuka
U.S. Small Businesses: Good News and Bad News November 21 2016 by Patrice Lee Onwuka
Arizona’s New Minimum Wage Packs a One-Two Punch for Nonprofits November 18 2016 by Patrice Lee Onwuka
Washington State Minimum Wage Passed and Childcare Costs Skyrocket November 17 2016 by Patrice Lee Onwuka
Hi, I’m Ben, Your Senator and Uber Driver! November 15 2016 by Patrice Lee Onwuka
Facebook Ends Multicultural Targeted Ads November 14 2016 by Patrice Lee Onwuka
Oprah’s Guarded Hope for the New President Triggers the Left November 14 2016 by Patrice Lee Onwuka
Star-Power Failed to Energize Millennials at the Polls November 10 2016 by Patrice Lee Onwuka
Mom-preneur Faces Charges for Selling Ceviche? November 9 2016 by Patrice Lee Onwuka
Uber is Back on the Road in Philly November 7 2016 by Patrice Lee Onwuka
Airbnb’s Self-Policing Strategy against Discrimination November 4 2016 by Patrice Lee Onwuka
Sanders and Warren Ignore that America is Better Off Now While Promoting Socialism • Evening Edit October 14 2019 by Patrice Lee Onwuka
Biden campaign plans to lose Iowa, New Hampshire, and Nevada • Rising October 11 2019 by Patrice Lee Onwuka
Is Warren trying to win black voters by floating Gillum as VP • Rising October 11 2019 by Patrice Lee Onwuka
US Businesses Should Stand Behind Hong Kong • Making Money October 9 2019 by Patrice Lee Onwuka
Left Leaning Outlets Turn on NYT Following Kavanaugh Hit Piece • Evening Edit September 23 2019 by Patrice Lee Onwuka
Partisan Party Politics Turns American Voters Off • Lou Dobbs Tonight September 18 2019 by Patrice Lee Onwuka
Bashing Billionaires Dissuades Everyone from Trying to Reach the Top • Making Money September 13 2019 by Patrice Lee Onwuka
Dems Have a Hard Case to Make Heading Into 2020 • Your World with Neil Cavuto September 9 2019 by Patrice Lee Onwuka
Dems Prioritize Gun Reform But Its All Talk • Evening Edit September 9 2019 by Patrice Lee Onwuka
Talking Tech: Is Being a "Big Company" a Bad Thing? • Making Money with Charles Payne September 6 2019 by Patrice Lee Onwuka
Media Needs to Find Balance Between Criticism and Support of President • Evening Edit September 3 2019 by Patrice Lee Onwuka
Democrats Continue to Focus on Emotion Over Fact: Biden and AOC • Trish Regan Primetime August 30 2019 by Patrice Lee Onwuka
Is the DNC rigging the debate rules? • Rising August 29 2019 by Patrice Lee Onwuka
Is South Carolina Biden's Hail Mary? • Rising August 29 2019 by Patrice Lee Onwuka
4 in 10 People Won't Trust the 2020 Election Results if Their Team Loses • Lou Dobbs Tonight August 28 2019 by Patrice Lee Onwuka
Taking Border Security Seriously with Approved Funding for The Wall • Evening Edit August 27 2019 by Patrice Lee Onwuka
Women Are Winning in This Economy • Coast to Coast August 22 2019 by Patrice Lee Onwuka
Language Police: In San Francisco and on Facebook • Lou Dobbs Tonight August 22 2019 by Patrice Lee Onwuka
Harry Reid Denounces Trajectory of Dem Party • Making Money with Charles Payne August 21 2019 by Patrice Lee Onwuka
Judge Trump’s actions, not words — and other commentary August 19 2019 by Patrice Lee Onwuka
Conservative commentator rips Shapiro over criticism of people with multiple jobs August 16 2019 by Patrice Lee Onwuka
How Will the Economy Effect the 2020 Conversation? • Coast to Coast August 15 2019 by Patrice Lee Onwuka
Israel Should Say "Thanks, But No Thanks" to Reps. Omar and Tlaib • Lou Dobbs Tonight August 15 2019 by Patrice Lee Onwuka
We Need to Act Now to Steer Away from Red Flags Ahead • Making Money August 14 2019 by Patrice Lee Onwuka
"In God We Trust" Is Important to Display in School • Lou Dobbs Tonight August 14 2019 by Patrice Lee Onwuka
Political Talking Points Stir Up Violent Threats Against SoulCycle Owner • Evening Edit August 9 2019 by Patrice Lee Onwuka
Pitting Consumers Against Businesses in the Name of Politics • Coast to Coast August 8 2019 by Patrice Lee Onwuka
Democratic Socialists Cannot Defeat Capitalism with Proper Pronouns and Complaints • Lou Dobbs August 6 2019 by Patrice Lee Onwuka
Don't pander to women; offer real opportunity August 1 2019 by Patrice Lee Onwuka
Bernie Ignores Legitimate Questions About his Health Care Plans • Evening Edit July 31 2019 by Patrice Lee Onwuka
The Washington Post Plays Scapegoat for Those Still Hung Up on 2016 Election • Lou Dobbs Tonight July 30 2019 by Patrice Lee Onwuka
Food Stamps Cut Because Booming Economy Means Less People Need Them • Fox & Friends First July 24 2019 by Patrice Lee Onwuka
The Squad Tries Pushing the Democrats Further Left Over Israel • Evening Edit July 24 2019 by Patrice Lee Onwuka
$15 Minimum Wage Robs Women of Jobs, Opportunity, Economic Mobility July 18 2019 by Patrice Lee Onwuka
"Squad" Pushes Democrat Party Closer to Socialism • Coast to Coast July 17 2019 by Patrice Lee Onwuka
"Woke Activism" is No Way to Get Things Done in Congress • Fox & Friends First July 15 2019 by Patrice Lee Onwuka
Cory Gardner, the women’s vote and religion: Western Conservative Summit takeaways July 14 2019 by Patrice Lee Onwuka
Democrats Have Placed All Their Eggs in the Identity Politics Basket • Evening Edit July 11 2019 by Patrice Lee Onwuka
AOC Needs to Tread Lightly Instead of Pointing Fingers at House Leadership • Your World July 11 2019 by Patrice Lee Onwuka
Why Restaurant Workers are Rising Up to Save Tipped Wages July 9 2019 by Patrice Lee Onwuka
Women's World Cup Champions Demand Equal Pay, Do the Numbers Add Up? • After The Bell July 8 2019 by Patrice Lee Onwuka
The Folly of Demanding Amazon’s Tax Returns • Liberty Watch Radio July 7 2019 by Patrice Lee Onwuka
Democrats are Obsessed Over the Past as the Candidates Take Aim at Each Other • Making Money July 5 2019 by Patrice Lee Onwuka
Supreme Court Reaches Verdict on 2020 Census Question • Your World with Neil Cavuto June 27 2019 by Patrice Lee Onwuka
Democrats Ignore Booming Economy and Foreshadow Recession • Evening Edit June 25 2019 by Patrice Lee Onwuka
Economists Determine the Value of "Free" Social Media Apps • Making Money June 24 2019 by Patrice Lee Onwuka
Race is a Central Issue for 2020, Biden is Learning the Hard Way • Coast to Coast June 20 2019 by Patrice Lee Onwuka
Hearing on Reparations Shows Just How Complicated This Issue Is • Trish Regan Primetime June 19 2019 by Patrice Lee Onwuka
President Trump Prioritizes Legal Immigrants During Border Crisis • Lou Dobbs Tonight June 18 2019 by Patrice Lee Onwuka
Lets Take an Objective Look at the Russian Interference Allegations • PoliticsNation June 15 2019 by Patrice Lee Onwuka
Liberals Distract Voters From Booming Economy with Social Issues • Lou Dobbs Tonight June 14 2019 by Patrice Lee Onwuka
Why Kamala Harris’ ‘Equal Pay’ Proposal Will Hurt, Not Help Women June 13 2019 by Patrice Lee Onwuka
Republicans and Democrats at odds on overtime pay June 13 2019 by Patrice Lee Onwuka
Deep Fake Videos Can Have Dangerous Repercussions • Evening Edit June 12 2019 by Patrice Lee Onwuka
Bernie Sanders Bashes Walmart Execs Claiming Minimum Wage Isn't High Enough • Coast to Coast June 5 2019 by Patrice Lee Onwuka
Mandated Paid Time Off Is Not Only Bad for Most Businesses, It's Bad for Many Women June 5 2019 by Patrice Lee Onwuka
Democrats Throw Money at Homelessness But the Situation Still Gets Worse, Why? • Lou Dobbs Tonight June 5 2019 by Patrice Lee Onwuka
Stossel: The Paid Leave Fairy Tale • Reason TV June 4 2019 by Patrice Lee Onwuka
Crisis at the Border and Draining the Swamp, Trust Trump to Create Change • Making Money May 31 2019 by Patrice Lee Onwuka
Kamala Harris’ New Proposal to Punish Companies Renews Wage Gap Debate • Bold Politics May 24 2019 by Patrice Lee Onwuka
Conflict in D.C. Does Effect Markets, But Consumers Continue to Spend • Coast to Coast May 23 2019 by Patrice Lee Onwuka
The Gender Wage Gap and What You Can Do About It May 22 2019 by Patrice Lee Onwuka
Top Dems Blow Any Chance of Bipartisan Reform • Evening Edit May 22 2019 by Patrice Lee Onwuka
Women's Organization Announces The 2019 Empowered Woman of the Year May 21 2019 by Patrice Lee Onwuka
Kamala Harris Threatens to Fine Businesses that Contribute to the "Pay Gap" • Fox & Friends May 21 2019 by Patrice Lee Onwuka
Low Birth Rates Could Have Devastating Economic Impacts • Fox & Friends May 16 2019 by Patrice Lee Onwuka
Policy analyst on falling birth rates: Rhetoric from the left contributing to 'change in norms' May 16 2019 by Patrice Lee Onwuka
Sanctuary Cities Putting Illegal Immigrants Ahead of Citizens • Lou Dobbs Tonight May 8 2019 by Patrice Lee Onwuka
Trump Should Look to Private Sector for Federal Reserve Board Nominees • Coast to Coast May 2 2019 by Patrice Lee Onwuka
Every Aspect of the Economy is Thriving From Jobs, Wages, To Taxes• Fox & Friends First April 29 2019 by Patrice Lee Onwuka
Bernie Struggles to Win Over Black Women • Evening Edit April 25 2019 by Patrice Lee Onwuka
Patrice Onwuka on Plastic Bag Bans, Net Neutrality and Michelle Obama • Americhicks April 23 2019 by Patrice Lee Onwuka
Many Americans Don't Care About the Mueller Report • Cavuto Live April 20 2019 by Patrice Lee Onwuka
Obama Playbook Will Not Be Enough to Get Joe Biden in the White House • Fox & Friends April 20 2019 by Patrice Lee Onwuka
Mueller Report Finally Available: What did it say and what is next? • WAMU April 18 2019 by Patrice Lee Onwuka
Opportunity Zones Create Economic Prosperity • Making Money with Charles Payne April 17 2019 by Patrice Lee Onwuka
Left Tries Desperately To Keep Collusion Narrative Alive • Coast to Coast April 15 2019 by Patrice Lee Onwuka
As support for reparations grow, so does pushback from some black Americans April 12 2019 by Patrice Lee Onwuka
Democrats Are Selective When Deciding Who is Innocent or Guilty • Fox & Friends Saturday April 6 2019 by Patrice Lee Onwuka
Being Fiscally Conservative Includes Being Smart on All Aspects of the Economy • Coast to Coast April 3 2019 by Patrice Lee Onwuka
Don't Fall for the Equal Pay Day Myth • Larry O'Connor Show April 2 2019 by Patrice Lee Onwuka
Presidential Hopefuls Playing Politics with Joe Biden's Victims • Evening Edit April 1 2019 by Patrice Lee Onwuka
Joe Biden's 2020 Doom • Cheddar Politics April 1 2019 by Patrice Lee Onwuka
Marco Rubio's New Family Leave Bill is a Budget Neutral Conservative Plan • Coast to Coast March 27 2019 by Patrice Lee Onwuka
The gender pay gap still exists - but it's shrinking, according to a new study March 27 2019 by Patrice Lee Onwuka
Millions of American off food stamps under President Trump • OANN March 23 2019 by Patrice Lee Onwuka
The Electoral College Matters, and Here's Why • The Evening Edit March 20 2019 by Patrice Lee Onwuka
Threats to Drag Out Investigation Despite No Evidence Of Collusion • The Evening Edit March 13 2019 by Patrice Lee Onwuka
A Legislative Unicorn: Paid Family Leave Without New Taxes or Entitlements March 12 2019 by Patrice Lee Onwuka
Congress considers parental leave programs March 12 2019 by Patrice Lee Onwuka
The Ivanka Trump effect: Why Republicans are now championing paid family leave, and what their new proposal looks like March 12 2019 by Patrice Lee Onwuka
Joni Ernst, Mike Lee roll out conservative paid parental leave idea March 12 2019 by Patrice Lee Onwuka
Raising the Topic of Paid Parental Leave March 12 2019 by Patrice Lee Onwuka
Republicans Propose Plan to Let Parents Use Social Security for Paid Family Leave After Having a Child March 12 2019 by Patrice Lee Onwuka
GOP senators unveil paid parental leave proposal March 12 2019 by Patrice Lee Onwuka
Support Choices That Women Make When Looking at Gender Pay Gap • Making Money with Charles Payne March 8 2019 by Patrice Lee Onwuka
Trump Previews 2020 in CPAC Speech • Fox and Friends First March 4 2019 by Patrice Lee Onwuka
Consumer Confidence Bouncing Back as Trump Looks Ahead to 2020 • Coast to Coast March 4 2019 by Patrice Lee Onwuka
Pelosi Should Second Guess New Member's Calls for Trump Impeachment • The Evening Edit February 27 2019 by Patrice Lee Onwuka
AOC Lives in Luxury Housing in D.C. Despite Her Views on Affordable Housing • Fox and Friends First February 21 2019 by Patrice Lee Onwuka
Bernie Attacks Someone Who Achieved the American Dream • Making Money with Charles Payne February 20 2019 by Patrice Lee Onwuka
Bernie Sanders Continues to Misrepresent the Reality of Socialism • The Evening Edit February 19 2019 by Patrice Lee Onwuka
Meet the Women Who Protested the Women’s March January 21 2019 by Patrice Lee Onwuka
Trump Putting Border Security on the Table with DACA Extension • America's News Headquarters January 19 2019 by Patrice Lee Onwuka
Women's March is Not Inclusive of All Ideas • Fox & Friends January 19 2019 by Patrice Lee Onwuka
USPS Relies on Amazon: What this means for consumers • Your World with Neil Cavuto December 5 2018 by Patrice Lee Onwuka
How Will Markets React to a Potential Trade Deal with China? • Coast to Coast November 29 2018 by Patrice Lee Onwuka
Streisand accuses Trump of misogyny while liberals turn on their own November 28 2018 by Patrice Lee Onwuka
What Pete Davidson’s SNL Apology Could Teach Us About Civility • Bold Politics November 16 2018 by Patrice Lee Onwuka
Is Amazon Guilty of Taking Part In Corporate Welfare? • The Evening Edit November 14 2018 by Patrice Lee Onwuka
'Economic damage' predicted after city levies big tax on hi-tech firms November 13 2018 by Patrice Lee Onwuka
Markets React to Midterm Election Results • Coast to Coast November 12 2018 by Patrice Lee Onwuka
Volatile Markets Will Only Marginally Influence Midterms • The Evening Edit October 26 2018 by Patrice Lee Onwuka
PETA Claims Milk is Racist • Trish Regan Primetime October 23 2018 by Patrice Lee Onwuka
How Will Booming Economy Be Affected by The Fed? • Coast to Coast October 17 2018 by Patrice Lee Onwuka
Kanye Brings Attention to Real Economic Issues • Coast to Coast October 11 2018 by Patrice Lee Onwuka
Will Kavanaugh Confirmation Drive Women Away from the GOP? Not Likely October 8 2018 by Patrice Lee Onwuka
Anti-Kavanaugh Harassment Tactics Overlooked by the Left • Coast to Coast October 4 2018 by Patrice Lee Onwuka
Statement on #CancelKavanaugh Protest: Activists Use Dr. Ford to Exploit Emotion Over a Deeply Personal Issue October 4 2018 by Patrice Lee Onwuka
Republican Women Explain Why They Stand By Brett Kavanaugh More Than Ever October 3 2018 by Patrice Lee Onwuka
Women Want Kavanaugh Confirmed if FBI Finds No Corroborating Evidence • FBN am October 3 2018 by Patrice Lee Onwuka
A Tale of Two Cities: A Snapshot of Washington D.C. On the Historic Kavanaugh-Ford Hearing Day September 27 2018 by Patrice Lee Onwuka
FBI Investigation Was an Ongoing Distraction During Hearings • After the Bell September 27 2018 by Patrice Lee Onwuka
'There's No Presumption of Innocence': Women Rally Both for and Against Kavanaugh September 27 2018 by Patrice Lee Onwuka
Dems Would Love for Trump to Fire Rosenstein • Fox & Friends First September 25 2018 by Patrice Lee Onwuka
Republicans Have Gone Out of Their Way to Try to Hear Blasey • Coast to Coast September 21 2018 by Patrice Lee Onwuka
Jacques: Sexism is a losing strategy September 14 2018 by Patrice Lee Onwuka
Good Trade Relations with China is Good for the Economy • Coast to Coast September 12 2018 by Patrice Lee Onwuka
Will Reforming SNAP Benefit Americans? • Bold Politics September 7 2018 by Patrice Lee Onwuka
Motivations Behind Trying to Flip a Russian Oligarch • Fox & Friends First September 3 2018 by Patrice Lee Onwuka
More Than Ever, Millennials Are Taking Care of Their Aging Family Members • Bold Life August 24 2018 by Patrice Lee Onwuka
Trump's Commitment to Diversity in the White House Continues After Omarosa • Andrew Wilkow Show August 22 2018 by Patrice Lee Onwuka
Statement: IWF Applauds Roll Back of Clean Power Plan August 22 2018 by Patrice Lee Onwuka
Omarosa’s Antics Won't Undermine Trump’s Progress for Black Americans • Afternoons with Cliff Kelley August 21 2018 by Patrice Lee Onwuka
NYT Publishes Pure Speculation on Mueller Investigation • Fox & Friends First August 20 2018 by Patrice Lee Onwuka
America IS Great: a lesson from real Immigrants • Fox & Friends August 18 2018 by Patrice Lee Onwuka
Harley-Davidson Staying in U.S. is Huge Victory • Coast to Coast August 17 2018 by Patrice Lee Onwuka
Is there a racial gender pay gap? • Bold Politics August 10 2018 by Patrice Lee Onwuka
Ocasio-Cortez Forgets Socialism Didn't Work in South America • Your World with Neil Cavuto August 9 2018 by Patrice Lee Onwuka
Rubio-Wagner family leave bill would draw from Social Security August 7 2018 by Patrice Lee Onwuka
Explaining the Economic Security for New Parents Act • KDKA August 3 2018 by Patrice Lee Onwuka
Rubio's New Paid Leave Bill is Great for Women • Fox & Friends First August 2 2018 by Patrice Lee Onwuka
Bernie Sanders "Medicare for All" plan would be a disaster • Your World with Neil Cavuto July 31 2018 by Patrice Lee Onwuka
How will new GDP numbers influence the midterm elections • Coast to Coast July 30 2018 by Patrice Lee Onwuka
New Bill Aims to Extend Trump Tax Cuts Beyond 2025 • Fox & Friends First July 25 2018 by Patrice Lee Onwuka
Sessions prepares students for diverse opinions at college • Making Money with Charles Payne July 24 2018 by Patrice Lee Onwuka
Student loan debt saddling non-saving Millennials July 24 2018 by Patrice Lee Onwuka
Only 4.9 Percent of People Have Two Jobs • Fox & Friends First July 19 2018 by Patrice Lee Onwuka
Companies thinking about intellectual theft when dealing with China • Coast to Coast July 18 2018 by Patrice Lee Onwuka
Cyber Security Needs to be Taken Seriously During Trump-Putin Summit • FBN am July 16 2018 by Patrice Lee Onwuka
What Do You Think Of Seattle's Plastic Straw Ban? • Bold Life July 6 2018 by Patrice Lee Onwuka
Hopeful About North Korean Cooperation • FBN am July 6 2018 by Patrice Lee Onwuka
#133 Reforms needed for children's television July 2 2018 by Patrice Lee Onwuka
How will Supreme Court nominations effect the primaries? • Coast to Coast June 29 2018 by Patrice Lee Onwuka
Trump is no excuse for intimidation of women June 28 2018 by Patrice Lee Onwuka
Protesters Want Media Attention, Not Policy Change • Your World with Neil Cavuto June 22 2018 by Patrice Lee Onwuka
Immigration bills will be hard to pass, but we can't ignore this issue • FBN AM June 20 2018 by Patrice Lee Onwuka
We should be concerned that foreign agents had access to Hillary's emails • Fox & Friends First June 15 2018 by Patrice Lee Onwuka
Free Trade Without Free Riders • Coast to Coast June 7 2018 by Patrice Lee Onwuka
Democrats Playing Politics in Primaries • Making Money with Charles Payne June 4 2018 by Patrice Lee Onwuka
IWF Statement on New Steel and Aluminum Tariffs May 31 2018 by Patrice Lee Onwuka
Trump had good reason to pull out of meeting with North Korea • Making Money with Charles Payne May 24 2018 by Patrice Lee Onwuka
Should Medical Marijuana Be Allowed in Classrooms? • Fox & Friends First May 21 2018 by Patrice Lee Onwuka
Media Bias After They Refuse to Accept Trump's Corrected Statement • Your World with Neil Cavuto May 17 2018 by Patrice Lee Onwuka
Lower Birth Rates: Student Debt, Societal Change, Technological Advances • The Intelligence Report May 17 2018 by Patrice Lee Onwuka
China's Relationship with North Korea Could Make All The Difference • FBN am May 16 2018 by Patrice Lee Onwuka
#127 Questioning the Status Quo: Black voters don't owe their vote to either party May 14 2018 by Patrice Lee Onwuka
Were there problems with Cohen's payments? • Coast to Coast May 10 2018 by Patrice Lee Onwuka
Removing "In God We Trust" to be Politically Correct • Fox & Friends First May 7 2018 by Patrice Lee Onwuka
Proof Mueller is Fishing for Evidence • FBN am May 7 2018 by Patrice Lee Onwuka
We Want to Ensure a Legitimate and Lawful Immigration Process • FBN am April 26 2018 by Patrice Lee Onwuka
Nancy Pelosi Chokes on "Crumbs" Comment Because It Just Isn't True • Coast to Coast April 25 2018 by Patrice Lee Onwuka
#123 Education Savings Accounts for Military Families Can Make All the Difference April 23 2018 by Patrice Lee Onwuka
Why are so many young adults choosing to stay at home? • BoldTV April 20 2018 by Patrice Lee Onwuka
Gov. Brown is Choosing Sanctuary over Safety • Risk & Reward April 19 2018 by Patrice Lee Onwuka
Comey has made enemies on both sides of the aisle • Risk & Reward April 19 2018 by Patrice Lee Onwuka
Bias training won't do much at Starbucks • Intelligence Report with Trish Reagan April 19 2018 by Patrice Lee Onwuka
Tax Cuts Will Play Major Role in Midterms • Coast to Coast April 5 2018 by Patrice Lee Onwuka
New Survey: What would Americans do for a 10% raise? • Fox & Friends First April 5 2018 by Patrice Lee Onwuka
Trump to rescind spending with a maneuver Clinton used nearly 20 years ago • FBN:am April 5 2018 by Patrice Lee Onwuka
Citizenship Question MUST be Answered Truthfully • Cavuto Live April 2 2018 by Patrice Lee Onwuka
#118 New Amendment Provides Protection Against the Discrimination of Pregnant Women April 2 2018 by Patrice Lee Onwuka
On the New Spending Bill: Entitlements Drive the National Debt • Risk & Reward March 23 2018 by Patrice Lee Onwuka
Regulations will Hinder Innovation on Social Media • Risk & Reward March 23 2018 by Patrice Lee Onwuka
White House Millennials Conference to Address Economy, Free Speech, and Opioids March 18 2018 by Patrice Lee Onwuka
In the #MeToo era, young conservative women look for their spot March 16 2018 by Patrice Lee Onwuka
Shake Ups in White House Happening for a Reason • FBN AM March 15 2018 by Patrice Lee Onwuka
Dems Will Not Continue to Embrace Middle America • Coast to Coast March 14 2018 by Patrice Lee Onwuka
We Don't Want to Abandon Free Trade • Cavuto Live March 10 2018 by Patrice Lee Onwuka
GOP takes on paid family leave March 10 2018 by Patrice Lee Onwuka
Issues in California are a Classic Case of Federalism • Risk & Reward March 9 2018 by Patrice Lee Onwuka
A Trade War is Going to Undermine Current Economic Progress • Coast to Coast March 5 2018 by Patrice Lee Onwuka
#113 What You Don't Know About Immigrants: A real immigrant's story March 2 2018 by Patrice Lee Onwuka
A conservative solution to paid leave • The National Discourse Podcast February 27 2018 by Patrice Lee Onwuka
CNN Pushes Agenda at Townhall • Risk & Reward February 23 2018 by Patrice Lee Onwuka
Americans Recognize the 2nd Amendment is Important to Protect • Coast to Coast February 20 2018 by Patrice Lee Onwuka
Republicans Produced Plan, Waiting for Action Across the Aisle • FBN AM February 13 2018 by Patrice Lee Onwuka
#MeToo and Working Women • PBS To The Contrary February 10 2018 by Patrice Lee Onwuka
Fundamentals of Our Economy Are Strong • Coast to Coast February 8 2018 by Patrice Lee Onwuka
Republicans Tired of Partisan Bickering • Coast to Coast January 31 2018 by Patrice Lee Onwuka
Unions Losing Battle Against Inevitable Future • The Intelligence Report January 25 2018 by Patrice Lee Onwuka
Did Schumer Cave on Gov Shut Down? • FBN am January 23 2018 by Patrice Lee Onwuka
Dems ‘Big Risk’ Not Worth It • Your World January 22 2018 by Patrice Lee Onwuka
Media Attacks Trump's Doctor • Risk & Reward January 19 2018 by Patrice Lee Onwuka
The Left Criticizing Tax Plan and Immigration • Risk & Reward January 12 2018 by Patrice Lee Onwuka
Concerns on Trade with China • Coast to Coast January 10 2018 by Patrice Lee Onwuka
Weighing in on Trump vs. Bannon • FBN AM January 5 2018 by Patrice Lee Onwuka
U.N. Funding Cut Good for Women • Forbes on Fox December 29 2017 by Patrice Lee Onwuka
Next Moves for the GOP • Coast to Coast December 26 2017 by Patrice Lee Onwuka
Saving Money After the Holidays • Fox & Friends First December 26 2017 by Patrice Lee Onwuka
Senate Passes Tax Bill • FBN AM December 20 2017 by Patrice Lee Onwuka
Are Black Women Leaving The Democratic Party to Become Independents? December 19 2017 by Patrice Lee Onwuka
Final GOP Tax Bill • Risk & Reward December 15 2017 by Patrice Lee Onwuka
Statement: Unnecessary internet regulation no more, FCC right to overturn net neutrality rules December 14 2017 by Patrice Lee Onwuka
Calls for Conyers to Resign • Risk & Reward Pt. 2 December 1 2017 by Patrice Lee Onwuka
Steinle's Killer Acquitted • Risk & Reward Pt. 1 December 1 2017 by Patrice Lee Onwuka
FCC Net Neutrality Vote Looms: How could things change? • The Big Picture December 1 2017 by Patrice Lee Onwuka
Time for Transparency: Taxpayer dollars used to protect Congressional misbehavior • Coast to Coast November 30 2017 by Patrice Lee Onwuka
More Bang for Your Buck • Fox & Friends First November 30 2017 by Patrice Lee Onwuka
Are some Dems willing to sacrifice protections for women to hold onto power? • Intelligence Report November 28 2017 by Patrice Lee Onwuka
Federal Judge blocks Trump's EO on Sanctuary Cities: Is Trump at a disadvantage? • Bulls & Bears November 25 2017 by Patrice Lee Onwuka
Has NFL commissioner lost control of the hemorrhaging league? • Bulls & Bears November 25 2017 by Patrice Lee Onwuka
Black Friday protests dwindle exposing this uncomfortable truth • Bulls & Bears November 25 2017 by Patrice Lee Onwuka
Sexual harassment is wrong no matter when it occurred and by whom • Varney & Co. November 24 2017 by Patrice Lee Onwuka
Bernie Sanders Blatantly Gives Bill Clinton, Al Franken a Pass on Sexual Harassment November 20 2017 by Patrice Lee Onwuka
There's Nothing Glamorous about “Auntie" Maxine Waters & Her “Impeach Him!” Chant November 17 2017 by Patrice Lee Onwuka
Twitter cracking down on Verified accounts • Fox & Friends First November 17 2017 by Patrice Lee Onwuka
'Saturday Night Live' says DNC is old and out of touch • Risk & Reward November 13 2017 by Patrice Lee Onwuka
Tax reform push: Will there be compromise? • FBN:AM November 13 2017 by Patrice Lee Onwuka
Are tech companies truly neutral platforms or do they reflect bias? • Fox & Friends First November 3 2017 by Patrice Lee Onwuka
Statement: GOP Tax Bill is a Win for Women November 2 2017 by Patrice Lee Onwuka
CNBC Slams DNC Chair Tom Perez, 'Get Off The Talking Points' On GOP Tax Plan November 2 2017 by Patrice Lee Onwuka
We cannot choose our history but we can choose not to repeat it • Risk & Reward October 31 2017 by Patrice Lee Onwuka
We're seeing the waning days of the NFL protests • Risk & Reward October 31 2017 by Patrice Lee Onwuka
This tax reform plan will help all workers • FBN:AM October 30 2017 by Patrice Lee Onwuka
West Wing Reads for 10/30/17 October 30 2017 by Patrice Lee Onwuka
Will any democrats get on board with tax reform? • FBN AM October 26 2017 by Patrice Lee Onwuka
What role is Bannon playing outside of the White House? • MSNBC Live October 23 2017 by Patrice Lee Onwuka
Podcast #98 Why sexual assault statistics on college campuses are misleading October 19 2017 by Patrice Lee Onwuka
Will new Trump/McConnell bromance last to get agenda through? • FBN AM October 17 2017 by Patrice Lee Onwuka
Hillary caring about "women's issues" a sham? • The Intelligence Report October 16 2017 by Patrice Lee Onwuka
More sexual harrassment allegations come out in Hollywood • Risk & Reward October 13 2017 by Patrice Lee Onwuka
Wait–Are Black Women Leaving The Democratic Party? October 11 2017 by Patrice Lee Onwuka
Eminem bashes Trump in new music video, but does it matter? • Coast To Coast October 11 2017 by Patrice Lee Onwuka
Statement: Abandoning The Clean Power Plan: A Destructive Regulatory Package October 10 2017 by Patrice Lee Onwuka
These four GOP Senators won't vote for Graham-Cassidy • FBN AM September 26 2017 by Patrice Lee Onwuka
These celebrities are joining in on the NFL anthem protests... • Risk & Reward September 26 2017 by Patrice Lee Onwuka
This sport is standing up for the National Anthem... • Risk & Reward September 25 2017 by Patrice Lee Onwuka
Does Graham-Cassidy healthcare bill have a chance? • FBN AM September 21 2017 by Patrice Lee Onwuka
Emmy's and NFL in ratings slide due to politics • Risk & Reward September 19 2017 by Patrice Lee Onwuka
A new left-wing strategy against Trump September 18 2017 by Patrice Lee Onwuka
Can Trump pull bi-partisan support for tax reform? • FBN AM September 13 2017 by Patrice Lee Onwuka
Is there an opportunity for bi-partisanship with recent devastations? • Coast To Coast September 11 2017 by Patrice Lee Onwuka
White House shake up doesn't mean the agenda stops • Risk & Reward August 18 2017 by Patrice Lee Onwuka
Let's consider moving Confederate monuments to museums • FBN AM August 17 2017 by Patrice Lee Onwuka
Trump holds Congress' feet to the fire on health care • Coast To Coast August 11 2017 by Patrice Lee Onwuka
Why diversity quotas may not be a good thing for companies • Intelligence Report August 7 2017 by Patrice Lee Onwuka
Left trying to label 'Death Wish' alt-right • Risk & Reward August 7 2017 by Patrice Lee Onwuka
Podcast #90: How the Internet is Giving Women Back Their Time August 2 2017 by Patrice Lee Onwuka
Will those who voted against repeal face tough constituent questions? • FBN AM July 28 2017 by Patrice Lee Onwuka
Brave move by John McCain with healthcare vote? • FBN AM July 28 2017 by Patrice Lee Onwuka
Jeff Sessions is yet another distraction from Trump agenda • Your World July 27 2017 by Patrice Lee Onwuka
Is getting a better education for your children racist? • Risk & Reward July 24 2017 by Patrice Lee Onwuka
Why democrats have failed to come up with plan to make Obamacare better • FBN AM July 20 2017 by Patrice Lee Onwuka
Should Congress forget healthcare repeal and move onto tax reform? • The Intelligence Report July 18 2017 by Patrice Lee Onwuka
Will Congress work through August recess? • Coast To Coast July 11 2017 by Patrice Lee Onwuka
Was it wrong for Ivanka to sit in at the G20? • Your World July 10 2017 by Patrice Lee Onwuka
Would media act differently if it was Chelsea Clinton at the G20? • Risk & Reward July 10 2017 by Patrice Lee Onwuka
Is the Trump administration good for the economy? • Fox 5 DC July 7 2017 by Patrice Lee Onwuka
Is Trump's agenda back on track? • FBN AM June 29 2017 by Patrice Lee Onwuka
Will the healthcare bill pass before July 4 recess? • FBN AM June 29 2017 by Patrice Lee Onwuka
People would be better served if we had a real healthcare market place • Risk & Reward June 27 2017 by Patrice Lee Onwuka
Black women work hard but can’t get ahead. This expert says conservatives have the solution. June 19 2017 by Patrice Lee Onwuka
Is Trump hurting himself when he tweets? • Coast To Coast June 7 2017 by Patrice Lee Onwuka
This is why you shouldn't jump on Larry Hogan for vetoing paid sick leave bill • Larry O'Connor Show May 26 2017 by Patrice Lee Onwuka
How much damage has been done to the Trump administration by the media? • FBN AM May 24 2017 by Patrice Lee Onwuka
Miss USA Slammed For Saying Healthcare Is A Privilege Rather Than A Right • Marc Cox May 16 2017 by Patrice Lee Onwuka
One Black Conservative Made It On Liberal List Of Beautiful Black Women April 25 2017 by Patrice Lee Onwuka
Essence’s “100 Woke Women” List: Where Are All The Conservatives? April 20 2017 by Patrice Lee Onwuka
Do Voters Actually Care About Trump's Tax Returns? • Coast To Coast April 17 2017 by Patrice Lee Onwuka
PBS teaching ISIS sympathy • Risk & Reward April 14 2017 by Patrice Lee Onwuka
Ivanka Trump Accepts Role as White House Advisor • Coast to Coast March 30 2017 by Patrice Lee Onwuka
Ryan Pulls Healthcare Bill Before Vote • MSNBC Live March 28 2017 by Patrice Lee Onwuka
Debate over Cost of Healthcare Overhaul ahead of CBO Score • Coast to Coast March 14 2017 by Patrice Lee Onwuka
A Day without a Woman • Making Money March 9 2017 by Patrice Lee Onwuka
Any Momentum Left from President Trump's Address? • MSNBC Live March 5 2017 by Patrice Lee Onwuka
Political double-speak: Who's line is it? • Risk & Reward February 23 2017 by Patrice Lee Onwuka
CNN may suffer from ‘PESD’ post-election stress disorder • Risk & Reward February 23 2017 by Patrice Lee Onwuka
WWE's Linda McMahon Confirmed To Head The Small Business Administration • Steve Gruber February 17 2017 by Patrice Lee Onwuka
Vice President Pence addressing right-to-life marchers in D.C. • Coast to Coast January 27 2017 by Patrice Lee Onwuka
Raising Minimum Wage 'Not Worth It' January 23 2017 by Patrice Lee Onwuka
Have Race Relations Improved Under Obama Presidency? • Tipping Point January 14 2017 by Patrice Lee Onwuka
If Republicans rush to repeal ObamaCare without a strategy to provide immediate relief to its victims, the ongoing collapse of insurance markets will continue • Coast To Coast January 9 2017 by Patrice Lee Onwuka
Sanctions A Political Move By Obama? • Risk & Reward December 30 2016 by Patrice Lee Onwuka
Be Careful Jumping On Consensus Bandwagon • Coast To Coast December 23 2016 by Patrice Lee Onwuka
Irony: Conway Facing Work-Life-Balance Criticism From Left After Accepting Position • Your World December 22 2016 by Patrice Lee Onwuka
Are Election Results Everyone's Fault But Democrats? • Coast To Coast December 9 2016 by Patrice Lee Onwuka
Controversy Over Trump's Stocks-But Do Americans Care? • Your World December 7 2016 by Patrice Lee Onwuka
Oprah's Message Of Hope Triggers The Left • Garrison November 17 2016 by Patrice Lee Onwuka
Is There A Double Standard With Protests Around Country? • Coast To Coast November 14 2016 by Patrice Lee Onwuka
AirBnB's Growing List Of Enemies + Halloween's Cultural Appropriation Police • Garrison October 20 2016 by Patrice Lee Onwuka
Forging a Generation of Opportunity October 20 2016 by Patrice Lee Onwuka
Political Correctness Plagues Halloween • Tipping Point October 18 2016 by Patrice Lee Onwuka
Democrats Relationship With Media In Question After Wikileaks Dump • Coast To Coast October 14 2016 by Patrice Lee Onwuka
Is IQ or EQ More Important In The Presidential Debates? • Your World September 20 2016 by Patrice Lee Onwuka
Are Self-Driving Cars A Good Idea + AirBnB Doesn't Drive Rent Higher • Garrison September 15 2016 by Patrice Lee Onwuka
Podcast #51 College Students: Don't Major In Debt! September 15 2016 by Patrice Lee Onwuka
Mylan's EpiPen Monopoly Created By Big Government • Tipping Point September 13 2016 by Patrice Lee Onwuka
Will Hillary Send More Troops Back Into Middle East? • Risk & Reward September 9 2016 by Patrice Lee Onwuka
Ad Spoofs 9/11 Attack • Risk & Reward September 9 2016 by Patrice Lee Onwuka
Mylan & Big Government On Hook For Rising EpiPen Costs • Your World September 2 2016 by Patrice Lee Onwuka
Did Kaepernick Right His Protest By Kneeling During Anthem? • Risk & Reward September 2 2016 by Patrice Lee Onwuka
Georgetown Offers Preferential Admission Status To Descendants Of Slaves • Risk & Reward September 1 2016 by Patrice Lee Onwuka
Labor Force Participation Rate Reaches 38 Year Low • Tipping Point August 5 2016 by Patrice Lee Onwuka
Can Trump Recover From His Comments About Slain Soldier's Parents? • Risk & Reward August 1 2016 by Patrice Lee Onwuka
Can Trump Close The Gap With Minorities? • Tipping Point June 10 2016 by Patrice Lee Onwuka
Should Salaries Be Transparent? • PBS Point Taken May 3 2016 by Patrice Lee Onwuka
Staying Too Long? Life Expectancy; Girls & Sex • To The Contrary April 23 2016 by Patrice Lee Onwuka
Equal Pay Day & Women in the Workplace • PBS To The Contrary April 16 2016 by Patrice Lee Onwuka
The Liberty Vote & How Government Hurts Opportunity • Stossel March 12 2016 by Patrice Lee Onwuka
Goods Marketed To Women Cost More Than Identical Items For Men • After The Bell December 23 2015 by Patrice Lee Onwuka
Feds know best ... even with cosmetics December 4 2015 by Patrice Lee Onwuka
Social Security disability fund gets dealt another blow November 17 2015 by Patrice Lee Onwuka
Gravity CEO Defends $70,000 Minimum Salary, Says it was the Right Decision August 11 2015 by Patrice Lee Onwuka
Baltimore Mom Toya Graham, Carly Fiorina, Salma Hayek • To The Contrary May 2 2015 by Patrice Lee Onwuka
When did a 'blue-collar' job become a bad thing? May 1 2015 by Patrice Lee Onwuka
End of women's colleges?/Women's Mosque of America • To the Contrary March 28 2015 by Patrice Lee Onwuka
The [Un]Affordable Care Act/TurboTax data breach • WIBC Garrison February 19 2015 by Patrice Lee Onwuka
Women in STEM, Women CEOS, Hate Crimes on Rise? • To The Contrary February 14 2015 by Patrice Lee Onwuka
Generation Left Behind: Millennial's pummeling financial situation• WIBC Garrison December 18 2014 by Patrice Lee Onwuka
Women & Midterm Elections; Female Marines; Lean Together • PBS To The Contrary October 31 2014 by Patrice Lee Onwuka
Women CEOs; Egg Freezing; Male Survivors of Sexual Assault • PBS To The Contrary October 18 2014 by Patrice Lee Onwuka
Patronizing? WH uses Emojis to make its economic case to Gen Y • The Rick Amato Show October 13 2014 by Patrice Lee Onwuka
Zero Common Sense: America's feverish culture of alarmism • Stossel October 2 2014 by Patrice Lee Onwuka
NFL Abuse Scandals; Hillary Clinton & Economy • PBS To The Contrary September 19 2014 by Patrice Lee Onwuka
Border Crisis: Cleaning up unintended consequences from bad policy • PBS To The Contrary July 13 2014 by Patrice Lee Onwuka
Should there be achievement benchmarks before teachers get tenure? • The Rick Amato Show June 16 2014 by Patrice Lee Onwuka
For ObamaCare to be fiscally solvent 40% of enrollees must be under 35 • Rick Amato Show April 22 2014 by Patrice Lee Onwuka
Tax Freedom Day: Stop working for Uncle Sam and start working for ourselves • 1310 KFKA AM Colorado April 21 2014 by Patrice Lee Onwuka
How are young Americans reacting to ObamaCare? • CBN Newswatch March 27 2014 by Patrice Lee Onwuka
March Madness: A desperate and expensive attempt to sell ObamaCare • The Rick Amato Show March 18 2014 by Patrice Lee Onwuka
Reports show ObamaCare tax on medical devices resulting in the loss of about thousands of jobs • AM Colorado February 24 2014 by Patrice Lee Onwuka
Lax hiring practices of ObamaCare navigators • Conservative Commandos Radio Show February 13 2014 by Patrice Lee Onwuka
Lee • Convicted Felons Hired to Administer ObamaCare & Personal Info • Rick Amato Show (02.04.14) February 4 2014 by Patrice Lee Onwuka
Lee • What to expect from Obama's 5th State of the Union • Arise Xchange (01.28.14) January 28 2014 by Patrice Lee Onwuka
Lee • Who are the ObamaCare Orphans? ACA Olympics ad blitz costs what? • AM Colorado (01.13.14) January 13 2014 by Patrice Lee Onwuka
Debra Messing, leave black Trump supporters alone September 12 2019 by Patrice Lee Onwuka
Kamala Harris Misleads Black Women on Faux Equal Pay Holiday August 26 2019 by Patrice Lee Onwuka
On Black Women’s Equal Pay Day, we don’t have to be victims August 22 2019 by Patrice Lee Onwuka
Why didn't any Democratic candidates tell the truth about the gender pay gap? August 5 2019 by Patrice Lee Onwuka
A $15 Minimum Wage Would Put More Than A Million Out Of Work — And Tlaib Wants To Hit Even More July 24 2019 by Patrice Lee Onwuka
Let Women Entrepreneurs Be Their Own Bosses July 15 2019 by Patrice Lee Onwuka
Polling Shows Fewer People Are Proud to Be American, but We All Should Be July 4 2019 by Patrice Lee Onwuka
Forget Free College, Let’s Talk About Apprenticeships June 26 2019 by Patrice Lee Onwuka
Trump Delivers Second Chances for Black Inmates Re-entering Society June 14 2019 by Patrice Lee Onwuka
Kamala Harris Plan to 'Fix' the Wage Gap is Sure to Undermine Working Women May 28 2019 by Patrice Lee Onwuka
Millennials like me waited to start families. Paid leave will let me spend time with them. May 26 2019 by Patrice Lee Onwuka
This is a great time to be a working mother May 12 2019 by Patrice Lee Onwuka
Black Americans don't need reparations, we need jobs April 10 2019 by Patrice Lee Onwuka
New Parents Act an Innovative, Conservative Paid Parental Leave Plan March 29 2019 by Patrice Lee Onwuka
For Women's History Month, celebrate the conservative women that the media ignore March 26 2019 by Patrice Lee Onwuka
How parental leave aids families March 12 2019 by Patrice Lee Onwuka
Jussie Smollett Isn't Biggest Setback for Blacks, Ignoring Progress Is March 1 2019 by Patrice Lee Onwuka
The tax cuts didn't steal your tax refund February 28 2019 by Patrice Lee Onwuka
Women's March Has Inclusivity Problem and It's Not Just Anti-Semitism January 17 2019 by Patrice Lee Onwuka
Women need criminal justice reform November 26 2018 by Patrice Lee Onwuka
Post-Election Analysis: In Congress, It’s New Gridlock, Same Old Divisions November 13 2018 by Patrice Lee Onwuka
Blacks Reject Left's Regressive Agenda, Choose Trump's Confidence October 30 2018 by Patrice Lee Onwuka
Debt shouldn't hold back Stacey Abrams, but her victimhood mentality might October 29 2018 by Patrice Lee Onwuka
Elizabeth Warren is the problem with the diversity victimhood mentality October 17 2018 by Patrice Lee Onwuka
Don't underestimate Kanye (and Trump) – Rapper could successfully deliver for forgotten black communities October 11 2018 by Patrice Lee Onwuka
I Support Survivors and Stand With Kavanaugh October 5 2018 by Patrice Lee Onwuka
SNAP reforms in the farm bill can improve lives for Americans September 5 2018 by Patrice Lee Onwuka
Why Omarosa’s antics will fail to undermine Trump’s progress for black Americans August 20 2018 by Patrice Lee Onwuka
Playing Victim Card Won't Win at Empowering Black Women August 7 2018 by Patrice Lee Onwuka
Marco Rubio's paid leave plan would help black moms August 2 2018 by Patrice Lee Onwuka
Don't Match Maxine Waters' Incivility With Disrespect July 13 2018 by Patrice Lee Onwuka
Brett Kavanaugh's Supreme Court nomination offers a chance to fight sexism against all women July 12 2018 by Patrice Lee Onwuka
Why women on the Left are not marching for all separated kids July 3 2018 by Patrice Lee Onwuka
In the era of streaming shows, kids' TV regulations are stuck in the 1990s June 23 2018 by Patrice Lee Onwuka
Juneteenth Is Over but Progress for Blacks Must Grow June 21 2018 by Patrice Lee Onwuka
Starbucks' anti-bias training may serve up more harm than good June 2 2018 by Patrice Lee Onwuka
Anti-Bias Training Won't Help Starbucks May 29 2018 by Patrice Lee Onwuka
Michelle Obama's myopic view of women is what's wrong with feminism May 7 2018 by Patrice Lee Onwuka
Blacks Owe No Allegiance to the Left April 30 2018 by Patrice Lee Onwuka
Gender and race shouldn't define your politics April 30 2018 by Patrice Lee Onwuka
'Diamond & Silk' offer chance for bipartisan push back on social media censorship April 14 2018 by Patrice Lee Onwuka
A Real Free Market Might Save Cab Drivers From Financial Ruin April 4 2018 by Patrice Lee Onwuka
It's past time for the mainstream media to add opposing voices April 4 2018 by Patrice Lee Onwuka
Hillary Clinton’s sorry apology is why she’s no champion for women March 22 2018 by Patrice Lee Onwuka
Increased Competition Best Treatment For High Prescription Drugs Costs March 21 2018 by Patrice Lee Onwuka
‘Black Panther’ Exposes Immigrant Resentment in the Black Community March 15 2018 by Patrice Lee Onwuka
An immigrant's request: Keep these things in mind when debating immigration reform February 14 2018 by Patrice Lee Onwuka
When Did Joblessness Stop Mattering to Black Leaders? February 7 2018 by Patrice Lee Onwuka
The Grammys' #MeToo moment signals a dangerous direction for the movement January 30 2018 by Patrice Lee Onwuka
Black Americans' Record Employment, Tax Cuts and More January 9 2018 by Patrice Lee Onwuka
Hollywood's #TimesUp movement has been compromised by progressives January 9 2018 by Patrice Lee Onwuka
When States Hike the Minimum Wage, Women Lose Jobs December 22 2017 by Patrice Lee Onwuka
Women Can Get Excited About Tax Reform. Here’s Why! December 21 2017 by Patrice Lee Onwuka
Black Lives Matter Boycott Will Hurt Those It Claims to Help December 11 2017 by Patrice Lee Onwuka
Don't shed any tears for John Conyers December 5 2017 by Patrice Lee Onwuka
Richard Cordray's departure should end the campaign against payday loans November 20 2017 by Patrice Lee Onwuka
I Am Thankful for How Tax Reform Makes the American Dream Possible November 19 2017 by Patrice Lee Onwuka
Democratic women in the Senate were all for these tax reform principles until Trump proposed them October 30 2017 by Patrice Lee Onwuka
Trump Tax Cuts Will Help Black-Owned Businesses Thrive October 27 2017 by Patrice Lee Onwuka
Give Christopher Columbus a break October 9 2017 by Patrice Lee Onwuka
Black Women Leaving the Democratic Party, Cracking the Base September 27 2017 by Patrice Lee Onwuka
Betsy DeVos Stands for the Rights of Victims September 13 2017 by Patrice Lee Onwuka
NFL protests need to take a knee September 8 2017 by Patrice Lee Onwuka
Economic Agenda Will Do More for Black People Than Statue Removal August 28 2017 by Patrice Lee Onwuka
Government Can Hate A Name, But Still Must Respect It August 14 2017 by Patrice Lee Onwuka
The Google Diversity Memo Should Start the Conversation — Not End It August 8 2017 by Patrice Lee Onwuka
Entrepreneurship Is the Real Black Girl Magic August 7 2017 by Patrice Lee Onwuka
Serena Williams Swings But Misses With Black Women’s Equal Pay Day August 4 2017 by Patrice Lee Onwuka
New York's crackdown on dog-walking licenses is only hurting low-income workers July 21 2017 by Patrice Lee Onwuka
Business should back Larry Hogan's veto of paid sick leave July 18 2017 by Patrice Lee Onwuka
Dispelling the White House pay gap myth July 10 2017 by Patrice Lee Onwuka
Maybe it's time for the White House Office of Girls and Women to go July 4 2017 by Patrice Lee Onwuka
Why UK millennials voting for socialism could happen here, too June 27 2017 by Patrice Lee Onwuka
Dear Internet: Don’t Carry The ‘F**k You 2016’ Movement Into 2017 [VIDEO] December 31 2016 by Patrice Lee Onwuka
How To Win The 'War On Christmas' On Campus December 26 2016 by Patrice Lee Onwuka
The Scariest Part Of Halloween Is The Cultural Appropriation Police October 13 2016 by Patrice Lee Onwuka
Don't Major In DEBT September 12 2016 by Patrice Lee Onwuka
It's Time For Social Security Reform July 26 2016 by Patrice Lee Onwuka
Congratulations Class of 2016, You Majored in Debt May 13 2016 by Patrice Lee Onwuka
Should Over-the-Top College Acceptance Letters Woo Students? March 29 2016 by Patrice Lee Onwuka
Why Are So Many Working Americans Still Dependent on Food Stamps? March 17 2016 by Patrice Lee Onwuka
Nursing Needs More Competition March 14 2016 by Patrice Lee Onwuka
Reese Witherspoon’s Oscar-Worthy Truth For Young Women February 27 2016 by Patrice Lee Onwuka
Takeaways: Overtime Pay June 4 2019 by Patrice Lee Onwuka
Policy Focus: Overtime Pay May 30 2019 by Patrice Lee Onwuka
Takeaways: Welfare Reform 2.0 September 7 2018 by Patrice Lee Onwuka
Policy Focus: Welfare Reform 2.0 August 13 2018 by Patrice Lee Onwuka
Takeaways: What Drives the Wage Gap? April 5 2018 by Patrice Lee Onwuka
Takeaways: Why Requiring Work & Community Engagement is Good for Medicaid and the Poor January 29 2018 by Patrice Lee Onwuka
Takeaways: How Tax Reform Will Help Americans December 18 2017 by Patrice Lee Onwuka
Fact Sheet: Tax Reform is a Women's Issue September 15 2017 by Patrice Lee Onwuka
Policy Focus: Your Ideas, Your Rights - Intellectual Property in the 21st Century June 25 2017 by Patrice Lee Onwuka